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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0008209,
umls-concept:C0087111,
umls-concept:C0185023,
umls-concept:C0205148,
umls-concept:C0237497,
umls-concept:C0242724,
umls-concept:C0242765,
umls-concept:C0376624,
umls-concept:C0441633,
umls-concept:C0600395,
umls-concept:C0871261,
umls-concept:C1314972,
umls-concept:C1563772,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1947904,
umls-concept:C1999228,
umls-concept:C2825781,
umls-concept:C2911692
|
| pubmed:issue |
1
|
| pubmed:dateCreated |
1999-4-28
|
| pubmed:abstractText |
Confocal scanning laser microscopy was used to observe the location of Escherichia coli O157:H7 on and within lettuce leaves. Sections of leaves (ca. 0.5 by 0.5 cm) were inoculated by submersion in a suspension of E. coli O157:H7 (ca. 10(7) to 10(8) CFU/ml) overnight at 7 degrees C. Fluorescein isothiocyanate-labeled antibody was used to visualize the attached bacteria. E. coli O157:H7 was found attached to the surface, trichomes, stomata, and cut edges. Three-dimensional volume reconstruction of interior portions of leaves showed that E. coli O157:H7 was entrapped 20 to 100 microm below the surface in stomata and cut edges. Agar plate culturing and microscopic observation indicated that E. coli O157:H7 preferentially attached to cut edges, as opposed to the intact leaf surface. Dual staining with fluorescein isothiocyanate-labeled antibody and propidium iodide was used to determine viability of cells on artificially contaminated lettuce leaves after treatment with 20 mg/liter chlorine solution for 5 min. Many live cells were found in stomata and on cut edges following chlorine treatment. E. coli O157:H7 did not preferentially adhere to biofilm produced by Pseudomonas fluorescens on the leaf surface. In contrast to E. coli O157:H7, Pseudomonas adhered to and grew mainly on the intact leaf surface rather than on the cut edges.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0362-028X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9921820-Bacterial Adhesion,
pubmed-meshheading:9921820-Bacteriological Techniques,
pubmed-meshheading:9921820-Biofilms,
pubmed-meshheading:9921820-Chlorine,
pubmed-meshheading:9921820-Colony Count, Microbial,
pubmed-meshheading:9921820-Disinfection,
pubmed-meshheading:9921820-Escherichia coli Infections,
pubmed-meshheading:9921820-Escherichia coli O157,
pubmed-meshheading:9921820-Humans,
pubmed-meshheading:9921820-Lettuce,
pubmed-meshheading:9921820-Microscopy, Confocal,
pubmed-meshheading:9921820-Pseudomonas fluorescens
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Attachment of Escherichia coli O157:H7 to lettuce leaf surface and bacterial viability in response to chlorine treatment as demonstrated by using confocal scanning laser microscopy.
|
| pubmed:affiliation |
Center for Food Safety and Quality Enhancement, Department of Food Science and Technology, University of Georgia, Athens 30602-2106, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|