Linked Life Data

9921416

Source:http://linkedlifedata.com/resource/pubmed/id/9921416

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1999-4-13
pubmed:abstractText
Interactions of the Na(+)-Ca2+ exchanger with small molecules on cell Ca2+ signaling were elucidated in Chinese hamster ovary (CHO) C1 cells, which transfected a control vector without any expression of the Na(+)-Ca2+ exchanger's gene while CHO CK1.4 cells transfected an expression vector encoding the bovine cardiac Na(+)-Ca2+ exchanger's cDNA, treated with lithium- or sodium-buffer medium respectively, by using L16(2)15 multifactorial orthogonal statistics and fura-2 fluorescence real-time imaging. In contrast to controls of Li(+)-treated C1 cells, the store-dependent Ca(2+)-influx (SDCI) was enhanced by either the Na(+)-Ca2+ exchanger, Na(+), 1-¿(beta-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl¿-1H-imidazole HCl (SK&F96365) or ouabain, and by interactions of the Na(+)-Ca2+ exchanger with either Na+, SK&F96365 or both SK&F96365 and ouabain; and ATP-induced Ca2+ release (AICR) was activated by SK&F96365 or Na+ alone, interactions of the Na(+)-Ca2+ exchanger with SK&F96365 or Na+, and an interaction between SK&F96365 and ouabain. The dramatic interaction of the Na(+)-Ca2+ exchanger with small molecules indicates that cell Ca2+ signaling is generated by inositol triphosphate (InsP3)-dependent pathways, allosteric effects of the G-protein coupled P2y&2u purinoceptor and multi-site recognition. Our findings provide meaningful clues for designing new strategies of cardiocerebral vascular oxidative diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0753-3322
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
459-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Interaction of the Na(+)-Ca2+ exchanger with small molecules on cell Ca2+ signaling.
pubmed:affiliation
Department of Anesthesiology, Zhong Shan Hospital, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't