rdf:type |
|
lifeskim:mentions |
umls-concept:C0008142,
umls-concept:C0027096,
umls-concept:C0086740,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0457166,
umls-concept:C0679058,
umls-concept:C1413980,
umls-concept:C1458283,
umls-concept:C1514562,
umls-concept:C1547699,
umls-concept:C1687052,
umls-concept:C1880098,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2700640
|
pubmed:issue |
1
|
pubmed:dateCreated |
1999-3-25
|
pubmed:databankReference |
|
pubmed:abstractText |
A gene encoding a chitin synthase with a myosin motor-like domain (csm1) was isolated from Pyricularia oryzae using a PCR fragment amplified from a fungal chitin synthase conserved region. The deduced amino acid sequence of csm1 is homologous to that of CsmA of Aspergillus nidulans (65% identity). The putative gene product of csm1 is consisted of the myosin motor-like domain and a chitin synthase domain as in A. nidulans csmA. The chitin synthase domain of its C-terminus was also homologous to Aspergillus fumigatus ChsE (61.4% identity) and Ustilago maydis Chs6 (48.6% identity) that encode class V chitin synthases. Northern analysis demonstrated that the csm1 was expressed throughout the mycelial growth of P. oryzae. This is the first report on the isolation of the gene encoding a class V chitin synthase with the myosin motor-like domain from P. oryzae.
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0378-1097
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
170
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
131-9
|
pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9919661-Amino Acid Sequence,
pubmed-meshheading:9919661-Ascomycota,
pubmed-meshheading:9919661-Base Sequence,
pubmed-meshheading:9919661-Blotting, Northern,
pubmed-meshheading:9919661-Blotting, Southern,
pubmed-meshheading:9919661-Chitin Synthase,
pubmed-meshheading:9919661-DNA, Fungal,
pubmed-meshheading:9919661-Genes, Fungal,
pubmed-meshheading:9919661-Introns,
pubmed-meshheading:9919661-Molecular Sequence Data,
pubmed-meshheading:9919661-Myosins,
pubmed-meshheading:9919661-Oryza sativa,
pubmed-meshheading:9919661-Plant Diseases,
pubmed-meshheading:9919661-Polymerase Chain Reaction,
pubmed-meshheading:9919661-Restriction Mapping,
pubmed-meshheading:9919661-Sequence Analysis, DNA
|
pubmed:year |
1999
|
pubmed:articleTitle |
Isolation of csm1 encoding a class V chitin synthase with a myosin motor-like domain from the rice blast fungus, Pyricularia oryzae.
|
pubmed:affiliation |
Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea.
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pubmed:publicationType |
Journal Article
|