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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-2-23
|
| pubmed:abstractText |
The sensitivity of mammalian cell lines to diphtheria toxin (DT) varies between species. Monkey (Mk) Vero cells are highly sensitive to DT, whereas rat and mouse (Ms) cells are resistant; hamster (Hm) cells display moderate DT sensitivity. The precursor of the Mk heparin-binding epidermal growth factor-like growth factor (proHB-EGF) functions as a DT receptor but the Ms proHB-EGF does not. In this study we have cloned, expressed, and characterized the Hm proHB-EGF/DT receptor. The expression of Hm proHB-EGF confers moderate DT sensitivity to normally DT-resistant mouse cells. The amino acid sequence of Hm preproHB-EGF shows that, overall, it more closely resembles the Ms preproHB-EGF sequence, except in the DT-binding region where it more closely resembles the Mk sequence. In the DT-binding region the Hm proHB-EGF sequence differs from the Mk proHB-EGF in only four amino acid residues (124, 126, 133, and 147); one of these residues, Ile133 in Mk proHB-EGF, has been previously reported to be important for DT binding and sensitivity. Analysis of Mk proHB-EGF mutants with residues substituted for Ile133 suggests that Asn133 in Hm proHB-EGF may be responsible for the moderate DT sensitivity of Hm proHB-EGF-expressing cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Diphtheria Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/heparin-binding EGF-like growth...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:day |
19
|
| pubmed:volume |
254
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
325-9
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9918837-Amino Acid Sequence,
pubmed-meshheading:9918837-Animals,
pubmed-meshheading:9918837-CHO Cells,
pubmed-meshheading:9918837-Cell Line,
pubmed-meshheading:9918837-Cercopithecus aethiops,
pubmed-meshheading:9918837-Cloning, Molecular,
pubmed-meshheading:9918837-Cricetinae,
pubmed-meshheading:9918837-Diphtheria Toxin,
pubmed-meshheading:9918837-Drug Resistance,
pubmed-meshheading:9918837-Gene Library,
pubmed-meshheading:9918837-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:9918837-Mice,
pubmed-meshheading:9918837-Molecular Sequence Data,
pubmed-meshheading:9918837-Rats,
pubmed-meshheading:9918837-Receptors, Cell Surface,
pubmed-meshheading:9918837-Recombinant Fusion Proteins,
pubmed-meshheading:9918837-Sequence Alignment,
pubmed-meshheading:9918837-Sequence Homology, Amino Acid,
pubmed-meshheading:9918837-Species Specificity,
pubmed-meshheading:9918837-Vero Cells
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Hamster diphtheria toxin receptor: a naturally occurring chimera of monkey and mouse HB-EGF precursors.
|
| pubmed:affiliation |
Department of Microbiology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas, 75235-9048, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|