| pubmed-article:9918836 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0596972 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0028128 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0205112 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C1801960 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0038836 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C1519941 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C1521743 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0181904 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C1704646 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0728873 | lld:lifeskim |
| pubmed-article:9918836 | lifeskim:mentions | umls-concept:C0126266 | lld:lifeskim |
| pubmed-article:9918836 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9918836 | pubmed:dateCreated | 1999-2-23 | lld:pubmed |
| pubmed-article:9918836 | pubmed:abstractText | Lucigenin has been widely used as a chemiluminescent substrate to monitor vascular superoxide (O*-2) formation. The validity of lucigenin for detection of O*-2 has been questioned because O*-2 is generated by lucigenin itself. It has been shown that the concentration of lucigenin is a critical parameter affecting the validity of this assay. In the present studies we evaluated a reduced concentration of lucigenin (5 microM) as a tool to quantify O*-2 production in vascular tissue. Lucigenin-induced effects on endothelial function were assessed by isometric tension recording of isolated aortic rings suspended in organ baths. The effects of lucigenin on O*-2 production were studied using spin trapping and electron spin resonance spectroscopy. Lucigenin at 250 microM but not at 5 microM caused a significant attenuation of endothelium-dependent relaxations to acetylcholine, which was prevented by pretreatment with superoxide dismutase. Spin-trapping studies revealed that lucigenin at 250 microM increased vascular O*-2 production several fold while 5 microM lucigenin did not stimulate O*-2 production. Inhibition of NO synthase by NG-momomethyl-l-arginine as well as the removal of the endothelium almost doubled lucigenin-derived chemiluminescence (LDCL), indicating that basal production of endothelium-derived NO depresses the baseline chemiluminescence signal. Thus, lucigenin at a concentration of 5 microM seems to be a sensitive and valid probe for assessing O*-2 in vascular tissue. It can also be used as an indirect probe to estimate basal vascular NO release. | lld:pubmed |
| pubmed-article:9918836 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9918836 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9918836 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9918836 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9918836 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:9918836 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:MeinertzTT | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:HarrisonD GDG | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:HineSS | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:MülschAA | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:MünzelTT | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:SperlingDD | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:SkatchkovM... | lld:pubmed |
| pubmed-article:9918836 | pubmed:author | pubmed-author:SindermannII | lld:pubmed |
| pubmed-article:9918836 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:9918836 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9918836 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:9918836 | pubmed:volume | 254 | lld:pubmed |
| pubmed-article:9918836 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9918836 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9918836 | pubmed:pagination | 319-24 | lld:pubmed |
| pubmed-article:9918836 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:9918836 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:9918836 | pubmed:articleTitle | Validation of lucigenin as a chemiluminescent probe to monitor vascular superoxide as well as basal vascular nitric oxide production. | lld:pubmed |
| pubmed-article:9918836 | pubmed:affiliation | Division of Cardiology, Department of Internal Medicine, University Hospital Eppendorf, Hamburg, Germany. | lld:pubmed |
| pubmed-article:9918836 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9918836 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:9918836 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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