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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-2-23
|
| pubmed:abstractText |
Lucigenin has been widely used as a chemiluminescent substrate to monitor vascular superoxide (O*-2) formation. The validity of lucigenin for detection of O*-2 has been questioned because O*-2 is generated by lucigenin itself. It has been shown that the concentration of lucigenin is a critical parameter affecting the validity of this assay. In the present studies we evaluated a reduced concentration of lucigenin (5 microM) as a tool to quantify O*-2 production in vascular tissue. Lucigenin-induced effects on endothelial function were assessed by isometric tension recording of isolated aortic rings suspended in organ baths. The effects of lucigenin on O*-2 production were studied using spin trapping and electron spin resonance spectroscopy. Lucigenin at 250 microM but not at 5 microM caused a significant attenuation of endothelium-dependent relaxations to acetylcholine, which was prevented by pretreatment with superoxide dismutase. Spin-trapping studies revealed that lucigenin at 250 microM increased vascular O*-2 production several fold while 5 microM lucigenin did not stimulate O*-2 production. Inhibition of NO synthase by NG-momomethyl-l-arginine as well as the removal of the endothelium almost doubled lucigenin-derived chemiluminescence (LDCL), indicating that basal production of endothelium-derived NO depresses the baseline chemiluminescence signal. Thus, lucigenin at a concentration of 5 microM seems to be a sensitive and valid probe for assessing O*-2 in vascular tissue. It can also be used as an indirect probe to estimate basal vascular NO release.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/10,10'-dimethyl-9,9'-biacridinium,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Acridines,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Pentetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:day |
19
|
| pubmed:volume |
254
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
319-24
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9918836-Acetylcholine,
pubmed-meshheading:9918836-Acridines,
pubmed-meshheading:9918836-Animals,
pubmed-meshheading:9918836-Aorta,
pubmed-meshheading:9918836-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:9918836-Endothelium, Vascular,
pubmed-meshheading:9918836-Hypercholesterolemia,
pubmed-meshheading:9918836-Luminescent Measurements,
pubmed-meshheading:9918836-Muscle, Smooth, Vascular,
pubmed-meshheading:9918836-Nitric Oxide,
pubmed-meshheading:9918836-Pentetic Acid,
pubmed-meshheading:9918836-Rabbits,
pubmed-meshheading:9918836-Reproducibility of Results,
pubmed-meshheading:9918836-Scintillation Counting,
pubmed-meshheading:9918836-Superoxide Dismutase,
pubmed-meshheading:9918836-Superoxides,
pubmed-meshheading:9918836-Vasodilation,
pubmed-meshheading:9918836-omega-N-Methylarginine
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Validation of lucigenin as a chemiluminescent probe to monitor vascular superoxide as well as basal vascular nitric oxide production.
|
| pubmed:affiliation |
Division of Cardiology, Department of Internal Medicine, University Hospital Eppendorf, Hamburg, Germany.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|