Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-2-8
|
| pubmed:abstractText |
We recently showed that brief complement inhibition induces accommodation of hamster cardiac transplants in nude rats. We have reconstituted nude rats carrying an accommodated xenograft with syngeneic CD4+ or CD8+ T cells to investigate the cellular mechanism of xenograft rejection. We show that CD4+ T cells can initiate xenograft rejection (10 +/- 1.7 days) by promoting production of IgG xenoreactive Abs (XAb). These XAb are able to activate complement as well as to mediate Ab-dependent cell-mediated cytotoxicity. Adoptive transfer of these XAb into naive nude rats provoked hyperacute xenograft rejection (38 +/- 13 min). The rejection was significantly (p < 0.001) delayed by cobra venom factor (CVF; 11 +/- 8 h in four of five cases) but was still more rapid than in control nude rats (3.3 +/- 0.5 days). CVF plus NK cell depletion further prolonged survival (>7 days in four of five cases; p < 0.01 vs CVF only). CD8+ T cell-reconstituted nude rats rejected their grafts later (19.4 +/- 5.8 days) and required a larger number of cells for transfer as compared with CD4+ T cell-reconstituted nude rats. However, second xenografts were rejected more rapidly than first xenografts in CD8+ T cell-reconstituted nude rats (9 +/- 2 days), indicating that the CD8+ T cells had been activated. This study demonstrates that CD4+ and CD8+ T cells can both reject xenografts. The CD4+ cells do so at least in part by generation of helper-dependent XAb that act by both complement-dependent and Ab-dependent cell-mediated cytotoxicity mechanisms; the CD8+ cells do so as helper-independent cytotoxic T cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
162
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1206-14
|
| pubmed:dateRevised |
2005-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9916754-Adoptive Transfer,
pubmed-meshheading:9916754-Animals,
pubmed-meshheading:9916754-Antibodies, Heterophile,
pubmed-meshheading:9916754-Blood Transfusion,
pubmed-meshheading:9916754-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9916754-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9916754-Cricetinae,
pubmed-meshheading:9916754-Graft Rejection,
pubmed-meshheading:9916754-Graft Survival,
pubmed-meshheading:9916754-Heart Transplantation,
pubmed-meshheading:9916754-Immunohistochemistry,
pubmed-meshheading:9916754-Male,
pubmed-meshheading:9916754-Mesocricetus,
pubmed-meshheading:9916754-Rats,
pubmed-meshheading:9916754-Rats, Nude,
pubmed-meshheading:9916754-Time Factors,
pubmed-meshheading:9916754-Transplantation, Heterologous
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Rejection of cardiac xenografts by CD4+ or CD8+ T cells.
|
| pubmed:affiliation |
Immunobiology Research Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
|
| pubmed:publicationType |
Journal Article
|