Linked Life Data

9915845

Source:http://linkedlifedata.com/resource/pubmed/id/9915845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-3-3
pubmed:databankReference
pubmed:abstractText
Protein kinase A-anchoring proteins (AKAPs) localize the second messenger response to particular subcellular domains by sequestration of the type II protein kinase A. Previously, AKAP120 was identified from a rabbit gastric parietal cell cDNA library; however, a monoclonal antibody raised against AKAP120 labeled a 350-kDa band in Western blots of parietal cell cytosol. Recloning has now revealed that AKAP120 is a segment of a larger protein, AKAP350. We have now obtained a complete sequence of human gastric AKAP350 as well as partial cDNA sequences from human lung and rabbit parietal cells. The genomic region containing AKAP350 is found on chromosome 7q21 and is multiply spliced, producing at least three distinct AKAP350 isoforms as well as yotiao, a protein associated with the N-methyl-D-aspartate receptor. Rabbit parietal cell AKAP350 is missing a sequence corresponding to a single exon in the middle of the molecule located just after the yotiao homology region. Two carboxyl-terminal splice variants were also identified. Both of the major splice variants showed tissue- and cell-specific expression patterns. Immunofluorescence microscopy demonstrated that AKAP350 was associated with centrosomes in many cell types. In polarized Madin-Darby canine kidney cells, AKAP350 localized asymmetrically to one pole of the centrosome, and nocodazole did not alter its localization. During the cell cycle, AKAP350 was associated with the centrosomes as well as with the cleavage furrow during anaphase and telophase. Several epithelial cell types also demonstrated noncentrosomal pools of AKAP350, especially parietal cells, which contained multiple cytosolic immunoreactive foci throughout the cells. The localization of AKAP350 suggests that it may regulate centrosomal and noncentrosomal cytoskeletal systems in many different cell types.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3055-66
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9915845-A Kinase Anchor Proteins, pubmed-meshheading:9915845-Adaptor Proteins, Signal Transducing, pubmed-meshheading:9915845-Alternative Splicing, pubmed-meshheading:9915845-Amino Acid Sequence, pubmed-meshheading:9915845-Animals, pubmed-meshheading:9915845-Base Sequence, pubmed-meshheading:9915845-Carrier Proteins, pubmed-meshheading:9915845-Cells, Cultured, pubmed-meshheading:9915845-Centrosome, pubmed-meshheading:9915845-Chromosomes, Human, Pair 7, pubmed-meshheading:9915845-Cloning, Molecular, pubmed-meshheading:9915845-Cyclic AMP-Dependent Protein Kinase Type II, pubmed-meshheading:9915845-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:9915845-Cytoskeletal Proteins, pubmed-meshheading:9915845-Dogs, pubmed-meshheading:9915845-Humans, pubmed-meshheading:9915845-Molecular Sequence Data, pubmed-meshheading:9915845-Parietal Cells, Gastric, pubmed-meshheading:9915845-Proteins, pubmed-meshheading:9915845-Rabbits
pubmed:year
1999
pubmed:articleTitle
AKAP350, a multiply spliced protein kinase A-anchoring protein associated with centrosomes.
pubmed:affiliation
Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta Veterans Affairs Medical Center, Augusta, Georgia 30912, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't