Linked Life Data

9914152

Source:http://linkedlifedata.com/resource/pubmed/id/9914152

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 4
pubmed:dateCreated
1999-4-27
pubmed:abstractText
When human platelets are stimulated with collagen or thrombin, the asymmetric distribution of membrane lipids is disrupted as phosphatidylserine and phosphatidylethanolamine translocate from the inner monolayer to the outer monolayer. Coincident with the stimulus-dependent rearrangement of membrane phospholipids is a rapid redistribution of cholesterol from the outer to the inner membrane monolayer. This redistribution of cholesterol was observed when the stimulus was collagen or ADP. The data presented here show that epinephrine stimulation does not promote cholesterol translocation but does potentiate collagen-promoted movement of cholesterol. To investigate the process of cholesterol translocation, experiments were performed to determine whether collagen stimulated reverse cholesterol movement; i.e. from the inner to the outer monolayer. For this study, the fluorescent sterol cholestatrienol (C-3) was incorporated into platelet membranes by exchange from cholesterol-containing phosphatidylcholine small unilamellar vesicles. C-3 was then removed selectively from the outer monolayer by treatment of the platelets with bovine serum albumin (BSA). During the subsequent incubation of BSA-treated platelets, C-3 moved spontaneously into the outer from the inner monolayer. This translocation had an apparent half-time of approximately 25 min and was unaltered by the presence of collagen. These results suggest that collagen treatment of platelets selectively facilitates the inward movement of the sterol. We have hypothesized that cholesterol translocation may be thermodynamically driven as a result of an unfavorable entropy, resulting in cholesterol translocation out of an environment becoming enriched in phosphatidylethanolamine. The unidirectional nature of collagen-promoted cholesterol movement from the phosphatidylethanolamine-rich outer monolayer is consistent with this interpretation.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-06, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-07, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-08, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-09, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-10A2, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-11, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-12, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-13A2, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-14, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-15, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-16, http://linkedlifedata.com/resource/pubmed/grant/R21 EY018705-01A1, http://linkedlifedata.com/resource/pubmed/grant/R21 EY018705-02, http://linkedlifedata.com/resource/pubmed/grant/R29 EY010420-03, http://linkedlifedata.com/resource/pubmed/grant/S10 RR026365-01
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-0949
pubmed:author
pubmed:issnType
Print
pubmed:volume
202
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
453-60
pubmed:dateRevised
2011-10-20
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Collagen-stimulated unidirectional translocation of cholesterol in human platelet membranes.
pubmed:affiliation
Department of Molecular Biology and Department of Cell Biology, UMDNJ School of Osteopathic Medicine, Stratford, NJ 08084, USA. battagli@UMDNJ.EDU.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't