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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-2-10
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pubmed:abstractText |
Triglycerides in the beta-cell may be important for stimulus-secretion coupling, through provision of a lipid-derived signal, and for pathogenetic events in NIDDM, where lipids may adversely affect beta-cell function. In adipose tissues, hormone-sensitive lipase (HSL) is rate-limiting in triglyceride hydrolysis. Here, we investigated whether this enzyme is also expressed and active in beta-cells. Northern blot analysis and reverse transcription-polymerase chain reaction demonstrated that HSL is expressed in rat islets and in the clonal beta-cell lines INS-1, RINm5F, and HIT-T15. Western blot analysis identified HSL in mouse and rat islets and the clonal beta-cells. In mouse and rat, immunocytochemistry showed a predominant occurrence of HSL in beta-cells, with a presumed cytoplasmic localization. Lipase activity in homogenates of the rodent islets and clonal beta-cells constituted 2.1 +/- 0.6% of that in adipocytes; this activity was immunoinhibited by use of antibodies to HSL. The established HSL expression and activity in beta-cells offer a mechanism whereby lipids are mobilized from intracellular stores. Because HSL in adipocytes is activated by cAMP-dependent protein kinase (PKA), PKA-regulated triglyceride hydrolysis in beta-cells may participate in the regulation of insulin secretion, possibly by providing a lipid-derived signal, e.g., long-chain acyl-CoA and diacylglycerol.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0012-1797
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
228-32
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9892250-Adipocytes,
pubmed-meshheading:9892250-Animals,
pubmed-meshheading:9892250-Blotting, Northern,
pubmed-meshheading:9892250-Clone Cells,
pubmed-meshheading:9892250-Enzyme Activation,
pubmed-meshheading:9892250-Fluorescent Antibody Technique,
pubmed-meshheading:9892250-Hydrolysis,
pubmed-meshheading:9892250-Islets of Langerhans,
pubmed-meshheading:9892250-Mice,
pubmed-meshheading:9892250-Mice, Inbred Strains,
pubmed-meshheading:9892250-Rats,
pubmed-meshheading:9892250-Rats, Sprague-Dawley,
pubmed-meshheading:9892250-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9892250-Sterol Esterase,
pubmed-meshheading:9892250-Triglycerides
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pubmed:year |
1999
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pubmed:articleTitle |
Hormone-sensitive lipase, the rate-limiting enzyme in triglyceride hydrolysis, is expressed and active in beta-cells.
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pubmed:affiliation |
Department of Cell and Molecular Biology, Lund University, Sweden. hindrik.mulder@medkem.lu.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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