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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-3-29
|
| pubmed:abstractText |
It is now generally accepted that massive neuronal death due to oxidative stress is a regular feature of brains in neurodegenerative diseases. However, much less attention has been given to the death of glial cells. In this study, we examined p53-sensitive apoptosis of cells by using human glioblastoma A172 cells and p53-deficient mouse astrocytes. In human A172 cells, hydrogen peroxide (H2O2) caused cell death in a time- and concentration-dependent manner, accompanied by nucleosomal DNA fragmentation and chromatin condensation. After treatment with H2O2, p53 protein was highly expressed and protein levels of Bak, p21WAF1/CIP1 and GADD45 were also enhanced. However, the protein levels of Bcl-2 and Bax did not change. On the other hand, primary cultured astrocytes from p53-deficient mouse brain grew faster than wild-type and heterozygous astrocytes. In addition, p53-deficient astrocytes were more resistant to H2O2-induced apoptosis than wild-type and heterozygous astrocytes. These results suggest that glial proliferation and the repair of damaged DNA may be regulated by p53-induced p21WAF1/CIP1 and GADD45, and that glial apoptosis caused by oxidative stress may be mediated by p53-induced Bak.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0894-1491
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
154-64
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9890630-Animals,
pubmed-meshheading:9890630-Apoptosis,
pubmed-meshheading:9890630-Brain Neoplasms,
pubmed-meshheading:9890630-Cell Survival,
pubmed-meshheading:9890630-DNA Damage,
pubmed-meshheading:9890630-DNA Fragmentation,
pubmed-meshheading:9890630-Glioblastoma,
pubmed-meshheading:9890630-Humans,
pubmed-meshheading:9890630-Hydrogen Peroxide,
pubmed-meshheading:9890630-In Situ Nick-End Labeling,
pubmed-meshheading:9890630-Male,
pubmed-meshheading:9890630-Mice,
pubmed-meshheading:9890630-Mice, Transgenic,
pubmed-meshheading:9890630-Microscopy, Electron,
pubmed-meshheading:9890630-Neuroglia,
pubmed-meshheading:9890630-Oxidants,
pubmed-meshheading:9890630-Oxidative Stress,
pubmed-meshheading:9890630-Tumor Cells, Cultured,
pubmed-meshheading:9890630-Tumor Suppressor Protein p53
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Hydrogen peroxide-induced apoptosis mediated by p53 protein in glial cells.
|
| pubmed:affiliation |
Department of Neurobiology, Kyoto Pharmaceutical University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|