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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Pt 1
|
| pubmed:dateCreated |
1999-3-9
|
| pubmed:abstractText |
We have identified an agent (SP-303) that shows efficacy against in vivo cholera toxin-induced fluid secretion and in vitro cAMP-mediated Cl- secretion. Administration of cholera toxin to adult mice results in an increase in fluid accumulation (FA) in the small intestine (FA ratio = 0.63 vs. 1.86 in control vs. cholera toxin-treated animals, respectively). This elevation in FA induced by cholera toxin was significantly reduced (FA ratio = 0.70) in animals treated with a 100 mg/kg dose of SP-303 at the same time as the cholera treatment. Moreover, when SP-303 was administered 3 h after cholera toxin, a dose-dependent inhibition of FA levels was observed with a half-maximal inhibitory dose of 10 mg/kg. In Ussing chamber studies of Caco-2 or T84 monolayer preparations, SP-303 had a significant effect on both basal current and forskolin-stimulated Cl- current. SP-303 also induced an increase in resistance that paralleled the observed decrease in current. These data suggest that SP-303 has an inhibitory effect on cAMP-mediated Cl- and fluid secretion. Thus SP-303 may prove to be a useful broad-spectrum antidiarrheal agent.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biopolymers,
http://linkedlifedata.com/resource/pubmed/chemical/Catechin,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/SP 303
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
276
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
G58-63
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9886979-Animals,
pubmed-meshheading:9886979-Biopolymers,
pubmed-meshheading:9886979-Body Fluids,
pubmed-meshheading:9886979-Caco-2 Cells,
pubmed-meshheading:9886979-Catechin,
pubmed-meshheading:9886979-Cell Line,
pubmed-meshheading:9886979-Chlorides,
pubmed-meshheading:9886979-Cholera Toxin,
pubmed-meshheading:9886979-Cyclic AMP,
pubmed-meshheading:9886979-Dose-Response Relationship, Drug,
pubmed-meshheading:9886979-Drug Administration Schedule,
pubmed-meshheading:9886979-Electric Conductivity,
pubmed-meshheading:9886979-Electric Impedance,
pubmed-meshheading:9886979-Female,
pubmed-meshheading:9886979-Forskolin,
pubmed-meshheading:9886979-Humans,
pubmed-meshheading:9886979-Intestine, Small,
pubmed-meshheading:9886979-Male,
pubmed-meshheading:9886979-Mice,
pubmed-meshheading:9886979-Mice, Inbred C57BL
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
A novel plant-derived inhibitor of cAMP-mediated fluid and chloride secretion.
|
| pubmed:affiliation |
Department of Pediatric Gastroenterology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|