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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1 Pt 1
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pubmed:dateCreated |
1999-3-9
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pubmed:abstractText |
Pancreatic dysfunction in patients with cystic fibrosis (CF) is felt to result primarily from impairment of ductal HCO-3 secretion. We provide molecular evidence for the expression of NBC-1, an electrogenic Na+-HCO-3 cotransporter (NBC) in cultured human pancreatic duct cells exhibiting physiological features prototypical of CF duct fragments (CFPAC-1 cells) or normal duct fragments [CAPAN-1 cells and CFPAC-1 cells transfected with wild-type CF transmembrane conductance regulator (CFTR)]. We further demonstrate that 1) HCO-3 uptake across the basolateral membranes of pancreatic duct cells is mediated via NBC and 2) cAMP potentiates NBC activity through activation of CFTR-mediated Cl- secretion. We propose that the defect in agonist-stimulated ductal HCO-3 secretion in patients with CF is predominantly due to decreased NBC-driven HCO-3 entry at the basolateral membrane, secondary to the lack of sufficient electrogenic driving force in the absence of functional CFTR.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C16-25
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9886916-Amino Acid Sequence,
pubmed-meshheading:9886916-Bicarbonates,
pubmed-meshheading:9886916-Carrier Proteins,
pubmed-meshheading:9886916-Cell Line,
pubmed-meshheading:9886916-Cell Membrane,
pubmed-meshheading:9886916-Cyclic AMP,
pubmed-meshheading:9886916-Cystic Fibrosis,
pubmed-meshheading:9886916-Cystic Fibrosis Transmembrane Conductance Regulator,
pubmed-meshheading:9886916-DNA, Complementary,
pubmed-meshheading:9886916-Drug Synergism,
pubmed-meshheading:9886916-Electric Conductivity,
pubmed-meshheading:9886916-Electrophysiology,
pubmed-meshheading:9886916-Humans,
pubmed-meshheading:9886916-Molecular Sequence Data,
pubmed-meshheading:9886916-Pancreatic Ducts,
pubmed-meshheading:9886916-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9886916-Sodium-Bicarbonate Symporters,
pubmed-meshheading:9886916-Tissue Distribution
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pubmed:year |
1999
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pubmed:articleTitle |
CFTR drives Na+-nHCO-3 cotransport in pancreatic duct cells: a basis for defective HCO-3 secretion in CF.
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pubmed:affiliation |
Division of Nephrology and Hypertension, Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0585, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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