Linked Life Data

9886375

Source:http://linkedlifedata.com/resource/pubmed/id/9886375

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-1-21
pubmed:abstractText
In the present study, we examined the structural requirements of peptide Ags for productive interactions with the TCR of CTL. For this purpose, we used as a model a previously identified immunodominant epitope that represents the target of EBV-specific HLA-A11-restricted CTL responses. By the use of peptides having minimal sequence homology with the wild-type epitope, we demonstrated that it is possible to selectively expand and reactivate memory CTL precursors without triggering the lytic mechanisms of wild-type specific effectors. In fact, stimulation of PBL from EBV-seropositive donors by polyalanine analogues, sharing only the putative TCR contact residue with the natural epitope, exclusively induced clonal expansion and reactivation of EBV-specific memory CTL precursors. Interestingly, these polyalanine peptides failed to trigger the cytotoxic function of CTLs specific for the wild-type viral epitope. This clearly indicates that reactivation of memory CTL precursors and triggering of the cytotoxic function have different requirements. The same phenomenon was observed using as stimulators naturally occurring peptides carrying the appropriate TCR contact residue. These data strongly suggest that cross-reactive peptides may play an important role in the expansion and reactivation of CTL clones from the memory T cell pool, and may be involved in long-term maintenance of T cell memory.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
106-13
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9886375-Amino Acid Substitution, pubmed-meshheading:9886375-Amino Acids, pubmed-meshheading:9886375-Cell Line, Transformed, pubmed-meshheading:9886375-Cross Reactions, pubmed-meshheading:9886375-Cytotoxicity, Immunologic, pubmed-meshheading:9886375-Epitopes, T-Lymphocyte, pubmed-meshheading:9886375-Epstein-Barr Virus Nuclear Antigens, pubmed-meshheading:9886375-Gene Products, pol, pubmed-meshheading:9886375-HIV, pubmed-meshheading:9886375-HLA-A Antigens, pubmed-meshheading:9886375-HLA-A11 Antigen, pubmed-meshheading:9886375-Humans, pubmed-meshheading:9886375-Immunologic Memory, pubmed-meshheading:9886375-Lymphocyte Activation, pubmed-meshheading:9886375-Oligopeptides, pubmed-meshheading:9886375-Peptides, pubmed-meshheading:9886375-Receptors, Antigen, T-Cell, pubmed-meshheading:9886375-Sequence Homology, Amino Acid, pubmed-meshheading:9886375-T-Lymphocyte Subsets, pubmed-meshheading:9886375-T-Lymphocytes, Cytotoxic, pubmed-meshheading:9886375-Tumor Cells, Cultured, pubmed-meshheading:9886375-Tumor Suppressor Protein p53
pubmed:year
1999
pubmed:articleTitle
A single specific amino acid residue in peptide antigens is sufficient to activate memory CTL: potential role of cross-reactive peptides in memory T cell maintenance.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Ferrara, Italy. reali.eva@hsr.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't