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rdf:type |
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lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0017262,
umls-concept:C0021760,
umls-concept:C0069676,
umls-concept:C0185117,
umls-concept:C0205227,
umls-concept:C0205263,
umls-concept:C0442805,
umls-concept:C0521447,
umls-concept:C0598894,
umls-concept:C1367477,
umls-concept:C1521761,
umls-concept:C2003941,
umls-concept:C2350701,
umls-concept:C2911684
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pubmed:issue |
12
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pubmed:dateCreated |
1999-3-24
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pubmed:abstractText |
Nuclear factor-IL-6 (NF-IL6) belongs to the CCAAT/enhancer binding protein family of transcription factors. NF-IL6 binds to the regulatory regions of many genes induced in activated macrophages in vitro. However, which particular genes are regulated by NF-IL6 in vivo is poorly defined. In order to identify the downstream genes of NF-IL6 in a monocytic lineage, we combined an inducible expression system with subtraction cloning in a study of murine M1 monocytic leukemia cells. We demonstrated that inducible expression of NF-IL6 is able to increase endogenous gene expression of macrophage inflammatory protein (MIP)-1 alpha, osteopontin and CD14 in M1 cells. We also showed that NF-IL6 activated murine MIP-1 alpha proximal promoter luciferase construct which contains two NF-IL6 binding sites and a point mutation of either site markedly reduces the luciferase activity. These findings indicate that MIP-1 alpha is a direct target of NF-IL6.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muramidase,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Osteopontin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SPP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spp1 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0953-8178
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1825-35
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9885903-Animals,
pubmed-meshheading:9885903-Antigens, CD14,
pubmed-meshheading:9885903-Base Sequence,
pubmed-meshheading:9885903-Binding Sites,
pubmed-meshheading:9885903-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:9885903-Chemokine CCL4,
pubmed-meshheading:9885903-DNA-Binding Proteins,
pubmed-meshheading:9885903-Gene Expression Regulation, Leukemic,
pubmed-meshheading:9885903-Humans,
pubmed-meshheading:9885903-Leukemia, Monocytic, Acute,
pubmed-meshheading:9885903-Macrophage Inflammatory Proteins,
pubmed-meshheading:9885903-Mice,
pubmed-meshheading:9885903-Molecular Sequence Data,
pubmed-meshheading:9885903-Muramidase,
pubmed-meshheading:9885903-Nuclear Proteins,
pubmed-meshheading:9885903-Osteopontin,
pubmed-meshheading:9885903-Promoter Regions, Genetic,
pubmed-meshheading:9885903-RNA, Messenger,
pubmed-meshheading:9885903-Sialoglycoproteins,
pubmed-meshheading:9885903-Transcriptional Activation,
pubmed-meshheading:9885903-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
Inducible expression of nuclear factor IL-6 increases endogenous gene expression of macrophage inflammatory protein-1 alpha, osteopontin and CD14 in a monocytic leukemia cell line.
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pubmed:affiliation |
Department of Biochemistry, Hyogo College of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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