Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1999-1-22
|
| pubmed:abstractText |
Interleukin-8 (IL-8) production by the gastric mucosa is increased in Helicobacter pylori infection. Previous studies indicated that H. pylori induces IL-8 synthesis in cancer cell lines, and the ability of H. pylori to stimulate IL-8 production is supposed to be associated with cagA and other cag pathogenicity island genes, including picB gene. In the present study, we investigated the induction of IL-8 in primary cultures of normal human gastric epithelial cells to elucidate the IL-8 induction by wild type strains and by the picB knockout strain. Human gastric epithelial cells were obtained from surgically resected specimens from four patients. Three H. pylori strains (TN2F4; type 1 clinical isolate, TN2F4m1; isogenic picB mutant of TN2F4, Tx30a; type 2 strain) were cocultured with the normal gastric epithelial cells or the transformed MKN-28. IL-8 levels in culture medium were determined by enzyme immunoassay. Human gastric epithelial cells produced IL-8 at a 10-50 times higher level than MKN-28 did when cocultured with TN2F4. The mutant TN2F4m1 induced IL-8 at significantly lower levels than the parent strain. Cells from four patients behaved similarly on IL-8 production. The results of the present study demonstrated the induction of IL-8 in normal gastric epithelial cells, suggesting that picB gene product may play an essential role in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0163-2116
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2738-43
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9881508-Bacterial Proteins,
pubmed-meshheading:9881508-Cells, Cultured,
pubmed-meshheading:9881508-Epithelial Cells,
pubmed-meshheading:9881508-Gastric Mucosa,
pubmed-meshheading:9881508-Helicobacter pylori,
pubmed-meshheading:9881508-Humans,
pubmed-meshheading:9881508-Interleukin-8,
pubmed-meshheading:9881508-Mutagenesis,
pubmed-meshheading:9881508-Virulence
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Interleukin-8 production in primary cultures of human gastric epithelial cells induced by Helicobacter pylori.
|
| pubmed:affiliation |
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|