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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1999-3-12
|
| pubmed:abstractText |
The levels of superoxide dismutase (SOD) were determined in detergent-soluble, somatic and excretion-secretion (E-S) preparations from adult Fasciola hepatica using the xanthine oxidase system and visualized in substrate gels. Compared to detergent-soluble and somatic extracts, E-S products showed the highest SOD activity (88.5 U/mg), indicating active release to the medium in which parasites were maintained. SOD specific activity was also detected at high levels in E-S products from 3-week-old and 5-week-old immature migrating flukes (25 and 143 U/mg, respectively). In all preparations except for the somatic extract, the activity was characterized as cyanide-sensitive CuZn SOD. Differences in SOD isoenzyme profiles between the extracts were observed in native polyacrylamide gel electrophoresis: the somatic and detergent-soluble extracts exhibited 1 band of activity while the E-S products from immature and adults flukes contained 2 and 3 migrating bands, respectively. SOD was purified from the detergent-soluble extract and E-S products of adult worms by a combination of ultrafiltration, gel filtration on Sephacryl S-200 HR and ion-exchange chromatography on QAE Sephadex A-50. The SOD from detergent-soluble extract showed, by SDS-PAGE analysis, 1 band of 16 kDa apparent molecular weight. The SOD from E-S products showed 2 bands of 16 and 60 kDa apparent molecular weight. N-terminal sequence analysis of the 16 kDa band from the detergent-soluble preparation showed some similarity with Schistosoma mansoni cytoplasmic SOD. These enzymes may have a potential role in the evasion of the oxidative burst killing mechanism by immune cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0031-1820
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
117 ( Pt 6)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
555-62
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9881380-Age Factors,
pubmed-meshheading:9881380-Animals,
pubmed-meshheading:9881380-Cattle,
pubmed-meshheading:9881380-Chromatography, Gel,
pubmed-meshheading:9881380-Chromatography, Ion Exchange,
pubmed-meshheading:9881380-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9881380-Fasciola hepatica,
pubmed-meshheading:9881380-Helminth Proteins,
pubmed-meshheading:9881380-Immunoblotting,
pubmed-meshheading:9881380-Male,
pubmed-meshheading:9881380-Rabbits,
pubmed-meshheading:9881380-Rats,
pubmed-meshheading:9881380-Rats, Wistar,
pubmed-meshheading:9881380-Superoxide Dismutase,
pubmed-meshheading:9881380-Ultrafiltration
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
CuZn superoxide dismutase activities from Fasciola hepatica.
|
| pubmed:affiliation |
Unidad de Biología Parasitaria, Facultad de Ciencias, Instituto de Higiene, Montevideo, Uruguay.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|