Linked Life Data

9881242

Source:http://linkedlifedata.com/resource/pubmed/id/9881242

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-1-26
pubmed:abstractText
One requirement prior to xenotransplantation of porcine islets during Type 1 diabetes is to eliminate the risk of transmitting infectious agents, particularly retroviruses, even when specific pathogen-free (SPF) pigs are used. We developed two sensitive complementary PCR-derived detection tests to assess this risk. This first is intended to detect a novel endogenous retrovirus pol sequence related to a recently described human endogenous retrovirus (HERV-L) and to foamy retroviruses. Primers for species-specific detection of this porcine endogenous sequence were designed and tested. The second test is a product-enhanced reverse transcriptase (PERT) assay optimised for the detection of porcine reverse transcriptase activity. These tests, which were used to detect HERV-L-related sequences and reverse transcriptase activity in certain SPF pig cells and porcine cell lines, may be useful in studying the risk of transmitting retroviruses by pig islet xenotransplantation in the immunogenetic context of Type 1 diabetes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1262-3636
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
434-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Detection of porcine endogenous retrovirus: possible involvement in pig islet xenotransplantation.
pubmed:affiliation
INRA/ENVN, University School of Medicine, Nantes, France. martignat@vet-nantes.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't