Linked Life Data

9880596

Source:http://linkedlifedata.com/resource/pubmed/id/9880596

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-2-16
pubmed:abstractText
To define the spatiotemporal development of and simultaneously select for oligodendrocytes (OLs) and Schwann cells (SCs), transgenic mice were generated that expressed a bacterial beta-galactosidase (beta-gal) and neomycin phosphotransferase fusion protein (betageo) under the control of murine 2'3'-cyclic nucleotide 3'-phosphodiesterase (muCNP) promoters I and II. Transgenic beta-gal activity was detected at embryonic day 12.5 in the ventral region of the rhombencephalon and spinal cord and in the neural crest. When cells from the rhombencephalon were cultured in the presence of G418, surviving cells differentiated into OLs, indicating that during development this brain region provides one source of OL progenitors. Postnatally, robust beta-gal activity was localized to OLs throughout the brain and was absent from astrocytes, neurons, and microglia or monocytes. In the sciatic nerve beta-gal activity was localized exclusively to SCs. Cultures from postnatal day 10 brain or sciatic nerve were grown in the presence of G418, and within 8-9 d exposure to antibiotic, 99% of all surviving cells were beta-gal-positive OLs or SCs. These studies demonstrate that the muCNP-betageo transgenic mice are useful for identifying OLs and SCs beginning at early stages of the glial cell lineage and throughout their development. This novel approach definitively establishes that the beta-gal-positive cells identified in vivo are glial progenitors, as defined by their ability to survive antibiotic selection and differentiate into OLs or SCs in vitro. Moreover, this experimental paradigm facilitates the rapid and efficient selection of pure populations of mouse OLs and SCs and further underscores the use of cell-specific promoters in the purification of distinct cell types.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
759-74
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9880596-3',5'-Cyclic-AMP Phosphodiesterases, pubmed-meshheading:9880596-Animals, pubmed-meshheading:9880596-Anti-Bacterial Agents, pubmed-meshheading:9880596-Blotting, Western, pubmed-meshheading:9880596-Culture Techniques, pubmed-meshheading:9880596-DNA, pubmed-meshheading:9880596-Drug Resistance, pubmed-meshheading:9880596-Genes, Reporter, pubmed-meshheading:9880596-Immunohistochemistry, pubmed-meshheading:9880596-Kanamycin Kinase, pubmed-meshheading:9880596-Mice, pubmed-meshheading:9880596-Mice, Transgenic, pubmed-meshheading:9880596-Neomycin, pubmed-meshheading:9880596-Neuroglia, pubmed-meshheading:9880596-Oligodendroglia, pubmed-meshheading:9880596-Recombinant Fusion Proteins, pubmed-meshheading:9880596-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:9880596-Schwann Cells, pubmed-meshheading:9880596-Selection, Genetic, pubmed-meshheading:9880596-Stem Cells, pubmed-meshheading:9880596-beta-Galactosidase
pubmed:year
1999
pubmed:articleTitle
Identification and characterization of early glial progenitors using a transgenic selection strategy.
pubmed:affiliation
National Institutes of Health, National Institute for Neurological Disorders and Stroke, Laboratory of Developmental Neurogenetics, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't