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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-2-16
|
| pubmed:abstractText |
We studied how Ca2+ influx through different subtypes of Ca2+ channels couples to release at a calyx-type terminal in the rat medial nucleus of the trapezoid body by simultaneously measuring the presynaptic Ca2+ influx evoked by a single action potential and the EPSC. Application of subtype-specific toxins showed that Ca2+ channels of the P/Q-, N-, and R-type controlled glutamate release at a single terminal. The Ca2+ influx through the P/Q-type channels triggered release more effectively than Ca2+ influx through N- or R-type channels. We investigated mechanisms that contributed to these differences in effectiveness. Electrophysiological experiments suggested that individual release sites were controlled by all three subtypes of Ca2+ channels. Immunocytochemical staining indicated, however, that a substantial fraction of N- and R-type channels was located distant from release sites. Although these distant channels contributed to the Ca2+ influx into the terminal, they may not contribute to release. Taken together, the results suggest that the Ca2+ influx into the calyx via N- and R-type channels triggers release less effectively than that via P/Q-type because a substantial fraction of the N- and R-type channels in the calyx is localized distant from release sites.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptotagmins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0270-6474
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
726-36
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9880593-Action Potentials,
pubmed-meshheading:9880593-Animals,
pubmed-meshheading:9880593-Brain Stem,
pubmed-meshheading:9880593-Calcium Channels,
pubmed-meshheading:9880593-Calcium-Binding Proteins,
pubmed-meshheading:9880593-Chelating Agents,
pubmed-meshheading:9880593-Egtazic Acid,
pubmed-meshheading:9880593-Electrophysiology,
pubmed-meshheading:9880593-Excitatory Postsynaptic Potentials,
pubmed-meshheading:9880593-Immunohistochemistry,
pubmed-meshheading:9880593-Kinetics,
pubmed-meshheading:9880593-Membrane Glycoproteins,
pubmed-meshheading:9880593-Nerve Tissue Proteins,
pubmed-meshheading:9880593-Neurotransmitter Agents,
pubmed-meshheading:9880593-Patch-Clamp Techniques,
pubmed-meshheading:9880593-Rats,
pubmed-meshheading:9880593-Rats, Wistar,
pubmed-meshheading:9880593-Synapses,
pubmed-meshheading:9880593-Synaptotagmins
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Calcium channel types with distinct presynaptic localization couple differentially to transmitter release in single calyx-type synapses.
|
| pubmed:affiliation |
Abteilung Zellphysiologie, Max-Planck-Institut für medizinische Forschung, D-69120 Heidelberg, Germany.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|