Linked Life Data

9877071

Source:http://linkedlifedata.com/resource/pubmed/id/9877071

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-2-8
pubmed:abstractText
In the present study, cell-surface markers and cytokine gene expression of lymphocytes from the local lymph nodes were studied 9 days after primary infection with Ostertagia ostertagi in previously naive calves or in calves previously immunized with multiple, chemically attenuated infections. Changes in lymphocyte populations were assessed by flow cytometry utilizing monoclonal antibodies specific for bovine cell-surface markers. Changes observed in the percentages of lymphoid populations after challenge were similar in animals immunized by either three or five drug-attenuated infections. In both immunized groups, the CD4+/CD8+ ratio was significantly higher than in naive animals after challenge infections. In addition, both immunized groups showed significantly lower levels of Ig-bearing cells upon experimental challenge when compared to animals with a primary experimental infection. No differences were observed in the number of gammadelta or interleukin 2 receptor (IL-2R) positive cells. The levels of mRNA for IL-4, IL-10, IL-15, IFN-gamma and TGF-beta1 were examined by competitive RT-PCR. After challenge, the levels of these cytokines were lower in animals immunized by five drug-attenuated infections, and in the case of IL-4 and TGF-beta1, these differences were statistically significant. These results indicate that animals exhibiting protection from reinfection with O. ostertagi do not show a shift to higher percentages of Ig+ cells characteristic of a primary infection. In addition, protected animals appear to show a decreased IL4 and TGF-beta1 response upon challenge when compared to non-immune animals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0304-4017
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-64
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9877071-Abomasum, pubmed-meshheading:9877071-Animals, pubmed-meshheading:9877071-Antibodies, Monoclonal, pubmed-meshheading:9877071-Antinematodal Agents, pubmed-meshheading:9877071-CD4-CD8 Ratio, pubmed-meshheading:9877071-Cattle, pubmed-meshheading:9877071-Cattle Diseases, pubmed-meshheading:9877071-Cytokines, pubmed-meshheading:9877071-DNA Primers, pubmed-meshheading:9877071-Fenbendazole, pubmed-meshheading:9877071-Flow Cytometry, pubmed-meshheading:9877071-Gene Expression Regulation, pubmed-meshheading:9877071-Immunization, pubmed-meshheading:9877071-Immunophenotyping, pubmed-meshheading:9877071-Interferon-gamma, pubmed-meshheading:9877071-Interleukin-10, pubmed-meshheading:9877071-Interleukin-15, pubmed-meshheading:9877071-Interleukin-4, pubmed-meshheading:9877071-Lymph Nodes, pubmed-meshheading:9877071-Ostertagia, pubmed-meshheading:9877071-Ostertagiasis, pubmed-meshheading:9877071-RNA, Messenger, pubmed-meshheading:9877071-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:9877071-Transforming Growth Factor beta
pubmed:year
1998
pubmed:articleTitle
Characterization of protective immune responses in local lymphoid tissues after drug-attenuated infections with Ostertagia ostertagi in calves.
pubmed:affiliation
USDA, ARS, LPSI, Immunology and Disease Resistance Laboratory, Beltsville, MD 20705, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't