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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1999-1-28
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pubmed:databankReference | |
pubmed:abstractText |
cDNA encoding N(G),N(G)-dimethylarginine dimethylaminohydrolase from rat kidney had been cloned [Kimoto, M., Sasakawa, T., Tsuji, H., Miyatake, S., Oka, T., Nio, N. & Ogawa, T. (1997) Biochim. Biophys. Acta 1337, 6-10]. The enzyme hydrolyzes N(G),N(G)-dimethyl-L-arginine and N(G)-monomethyl-L-arginine, which are known as endogenous inhibitors for the nitric oxide-generating system. In the present study, human N(G),N(G)-dimethylarginine dimethylaminohydrolase has been purified to homogeneity from liver and characterized. The cDNA clone encoding human N(G),N(G)-dimethylarginine dimethylaminohydrolase was isolated from a human kidney lambda gt10 library using a probe prepared from a plasmid containing the entire coding region of rat N(G),N(G)-dimethylarginine dimethylaminohydrolase. Its open reading frame encoded a protein of 285 amino acids with a molecular mass of 31,121 Da. The deduced amino acid sequence exhibits 93% identity with that of rat. The cDNA was expressed as a fusion protein in Escherichia coli and the recombinant protein exhibited enzyme activity which is the same as that of natural enzyme.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amidohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/dimethylargininase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
863-8
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:9874257-Amidohydrolases,
pubmed-meshheading:9874257-Amino Acid Sequence,
pubmed-meshheading:9874257-Base Sequence,
pubmed-meshheading:9874257-Cloning, Molecular,
pubmed-meshheading:9874257-Humans,
pubmed-meshheading:9874257-Hydrolases,
pubmed-meshheading:9874257-Kinetics,
pubmed-meshheading:9874257-Liver,
pubmed-meshheading:9874257-Molecular Sequence Data,
pubmed-meshheading:9874257-RNA, Messenger,
pubmed-meshheading:9874257-Recombinant Proteins,
pubmed-meshheading:9874257-Sequence Analysis, DNA,
pubmed-meshheading:9874257-Sequence Homology, Amino Acid,
pubmed-meshheading:9874257-Trypsin
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pubmed:year |
1998
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pubmed:articleTitle |
Purification, cDNA cloning and expression of human NG,NG-dimethylarginine dimethylaminohydrolase.
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pubmed:affiliation |
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja, Japan. kimoto@fhw.oka-pu.ac.jp
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pubmed:publicationType |
Journal Article
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