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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-2-22
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pubmed:databankReference |
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pubmed:abstractText |
Molecular studies of some micro-organisms are hampered by the difficulty of obtaining sufficient amounts of nucleic acids. A cloning strategy based on PCR has therefore been used to clone the eburicol 14alpha-demethylase (CYP51) gene of the obligate fungus Erysiphe graminis f. sp. hordei (Egh) using minute amounts of genomic DNA. The CYP51 gene encodes the enzymatic target of a major group of fungicides. Sequencing CYP51 from different Egh isolates revealed the occurrence of two alleles for this gene. An allele-specific PCR assay was developed to detect each CYP51 allele.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0172-8083
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
399-403
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9871123-Alleles,
pubmed-meshheading:9871123-Amino Acid Sequence,
pubmed-meshheading:9871123-Ascomycota,
pubmed-meshheading:9871123-Cloning, Molecular,
pubmed-meshheading:9871123-Cytochrome P-450 Enzyme System,
pubmed-meshheading:9871123-DNA, Complementary,
pubmed-meshheading:9871123-DNA, Fungal,
pubmed-meshheading:9871123-Gene Amplification,
pubmed-meshheading:9871123-Genes, Fungal,
pubmed-meshheading:9871123-Genetic Variation,
pubmed-meshheading:9871123-Molecular Sequence Data,
pubmed-meshheading:9871123-Oxidoreductases,
pubmed-meshheading:9871123-Point Mutation,
pubmed-meshheading:9871123-Polymerase Chain Reaction,
pubmed-meshheading:9871123-Sequence Alignment,
pubmed-meshheading:9871123-Sequence Homology, Amino Acid,
pubmed-meshheading:9871123-Sterol 14-Demethylase
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pubmed:year |
1998
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pubmed:articleTitle |
PCR cloning and detection of point mutations in the eburicol 14alpha-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a "recalcitrant" fungus.
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pubmed:affiliation |
Unité de Recherches Intégrées sur la Vigne, Institut National de la Recherche Agronomique, Domaine de la Grande Ferrade, B. P. 81, F-33883 Villenave d'Ornon cédex, France. delye@bordeaux.inra.fr
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pubmed:publicationType |
Journal Article
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