rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
4
|
pubmed:dateCreated |
1999-6-21
|
pubmed:abstractText |
We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0301-4851
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
25
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
231-6
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:9870613-Animals,
pubmed-meshheading:9870613-Cell Line,
pubmed-meshheading:9870613-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:9870613-Fibroblasts,
pubmed-meshheading:9870613-Hepatitis B virus,
pubmed-meshheading:9870613-Nuclear Proteins,
pubmed-meshheading:9870613-Precipitin Tests,
pubmed-meshheading:9870613-Promoter Regions, Genetic,
pubmed-meshheading:9870613-Protein Synthesis Inhibitors,
pubmed-meshheading:9870613-Rats,
pubmed-meshheading:9870613-Tetracycline,
pubmed-meshheading:9870613-Trans-Activators,
pubmed-meshheading:9870613-Transcription, Genetic,
pubmed-meshheading:9870613-Transcriptional Activation
|
pubmed:year |
1998
|
pubmed:articleTitle |
Transactivation by expression of the hepatitis B virus X protein with an inducible system.
|
pubmed:affiliation |
Moffitt Cancer Center & Research Institute, Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa 33612, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|