Linked Life Data

9869407

Source:http://linkedlifedata.com/resource/pubmed/id/9869407

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-1-15
pubmed:abstractText
Genetically engineered rice (Oryza sativa L.) with the ability to synthesize glycinebetaine was established by introducing the codA gene for choline oxidase from the soil bacterium Arthrobacter globiformis. Levels of glycinebetaine were as high as 1 and 5 micromol per gram fresh weight of leaves in two types of transgenic plant in which choline oxidase was targeted to the chloroplasts (ChlCOD plants) and to the cytosol (CytCOD plants), respectively. Although treatment with 0.15 M NaCl [corrected] inhibited the growth of both wild-type and transgenic plants, the transgenic plants began to grow again at the normal rate after a significantly less time than the wild-type plants after elimination of the salt stress. Inactivation of photosynthesis, used as a measure of cellular damage, indicated that ChlCOD plants were more tolerant than CytCOD plants to photoinhibition under salt stress and low-temperature stress. These results indicated that the subcellular compartmentalization of the biosynthesis of glycinebetaine was a critical element in the efficient enhancement of tolerance to stress in the engineered plants.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1011-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9869407-Acclimatization, pubmed-meshheading:9869407-Alcohol Oxidoreductases, pubmed-meshheading:9869407-Betaine, pubmed-meshheading:9869407-Chloroplasts, pubmed-meshheading:9869407-Cold Temperature, pubmed-meshheading:9869407-Cytosol, pubmed-meshheading:9869407-Gene Transfer Techniques, pubmed-meshheading:9869407-Genetic Engineering, pubmed-meshheading:9869407-Oryza sativa, pubmed-meshheading:9869407-Osmolar Concentration, pubmed-meshheading:9869407-Photosynthetic Reaction Center Complex Proteins, pubmed-meshheading:9869407-Plants, Genetically Modified, pubmed-meshheading:9869407-Plasmids, pubmed-meshheading:9869407-Recombinant Proteins, pubmed-meshheading:9869407-Restriction Mapping, pubmed-meshheading:9869407-Rhizobium, pubmed-meshheading:9869407-Transformation, Genetic
pubmed:year
1998
pubmed:articleTitle
Metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and cold.
pubmed:affiliation
National Institute for Basic Biology, Myodaiji, Okazaki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't