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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1999-1-28
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pubmed:abstractText |
Parasporal inclusions of a Bacillus thuringiensis isolate designated 92-KU-105-9 (H14/19) exhibited unusual larvicidal activity, specific for the moth-fly, Telmatoscopus albipunctatus (Diptera: Psychodidae), similar to that of a previously reported B. thuringiensis serovar leesis (H33) strain. The LC50 value of the purified inclusions was 4.92 micrograms ml-1 for the moth-fly larvae, while no mortality was shown in the mosquitoes Culex pipiens molestus and Anopheles stephensi, at protein concentrations up to 10 mg ml-1. Morphologically, the inclusion was a homogeneous globular body surrounded by an electron-dense, thick envelope. Multilamellar inner structure was evident between envelope membrane and inclusion matrix. SDS-PAGE revealed that the inclusions consist of five proteins with molecular masses of 72, 70, 68, 56 and 30 kDa. These proteins cross-reacted with the antibodies against inclusion proteins of the serovar leesis strain. High homologies existed in N-terminal amino acid sequences between the three major proteins (72, 70 and 68 kDa) and the two established protein classes, Cry4A and Cry10A.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
213-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9868764-Amino Acid Sequence,
pubmed-meshheading:9868764-Animals,
pubmed-meshheading:9868764-Bacillus thuringiensis,
pubmed-meshheading:9868764-Bacterial Proteins,
pubmed-meshheading:9868764-Humans,
pubmed-meshheading:9868764-Inclusion Bodies,
pubmed-meshheading:9868764-Microscopy, Electron,
pubmed-meshheading:9868764-Molecular Sequence Data,
pubmed-meshheading:9868764-Moths,
pubmed-meshheading:9868764-Pest Control, Biological,
pubmed-meshheading:9868764-Psychodidae,
pubmed-meshheading:9868764-Sequence Homology, Amino Acid,
pubmed-meshheading:9868764-Species Specificity
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pubmed:year |
1998
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pubmed:articleTitle |
Similarity in moth-fly specific larvicidal activity between two serologically unrelated Bacillus thuringiensis strains.
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pubmed:affiliation |
Section of Applied Microbiology, Fukuoka Industrial Technology Center, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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