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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0007634,
umls-concept:C0012120,
umls-concept:C0018270,
umls-concept:C0205314,
umls-concept:C0242210,
umls-concept:C0596235,
umls-concept:C0679058,
umls-concept:C0679622,
umls-concept:C1413374,
umls-concept:C1413375,
umls-concept:C1418757,
umls-concept:C1422407,
umls-concept:C1511938,
umls-concept:C1514559,
umls-concept:C1547699,
umls-concept:C1705165,
umls-concept:C2700061,
umls-concept:C2700640
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pubmed:issue |
6
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pubmed:dateCreated |
1999-3-8
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pubmed:abstractText |
Among the expressed genes associated with the switch-over of Dictyostelium cells from cell proliferation to differentiation, the Calfumirin-1 (CAF1) gene has been shown to be preferentially expressed at the initial step of differentiation, encoding a novel Ca2+-binding protein (Abe & Maeda 1995). To analyze precisely the function of CAF1, transformants overexpressing the CAF1 mRNA at the vegetative growth phase and also CAF1-null mutants were prepared, and their developmental features were compared with those of parental wild-type cells. As a result, the CAF1-overexpression was found to promote cell differentiation, possibly through prompt induction of the cAMP receptor 1 (CAR1) gene expression. In addition, the CAF1-overexpressing cells were able to differentiate even under low external Ca2+ ([Ca2+]e) conditions around 10(-6) mol/L at which non-transformed wild-type cells never differentiated. Unexpectedly, however, the CAF1-null mutant produced by homologous recombination exhibited apparently normal development to form fruiting bodies on non-nutrient agar. These results seem to indicate that CAF1-overexpression has a stimulatory effect on differentiation, but that the CAF1 protein is not necessarily required for the phase-shift of cells from growth to differentiation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0012-1592
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
677-83
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9865978-Animals,
pubmed-meshheading:9865978-Blotting, Northern,
pubmed-meshheading:9865978-Calcium,
pubmed-meshheading:9865978-Calcium-Binding Proteins,
pubmed-meshheading:9865978-Cell Differentiation,
pubmed-meshheading:9865978-Cell Division,
pubmed-meshheading:9865978-Dictyostelium,
pubmed-meshheading:9865978-Fungal Proteins,
pubmed-meshheading:9865978-Gene Expression,
pubmed-meshheading:9865978-Genetic Vectors,
pubmed-meshheading:9865978-Mutation,
pubmed-meshheading:9865978-RNA,
pubmed-meshheading:9865978-Receptors, Cyclic AMP,
pubmed-meshheading:9865978-Transcription Factors,
pubmed-meshheading:9865978-Transformation, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
Overexpression of CAF1 encoding a novel Ca2+-binding protein stimulates the transition of Dictyostelium cells from growth to differentiation.
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pubmed:affiliation |
Biological Institute, Graduate School of Science, Tohoku University, Aoba, Sendai, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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