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rdf:type |
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lifeskim:mentions |
umls-concept:C0014834,
umls-concept:C0015576,
umls-concept:C0040815,
umls-concept:C0219220,
umls-concept:C0444626,
umls-concept:C0680022,
umls-concept:C1514562,
umls-concept:C1704241,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
12
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pubmed:dateCreated |
1999-3-3
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pubmed:databankReference |
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pubmed:abstractText |
The crystal structure of the Escherichia coli trehalose repressor (TreR) in a complex with its inducer trehalose-6-phosphate was determined by the method of multiple isomorphous replacement (MIR) at 2.5 A resolution, followed by the structure determination of TreR in a complex with its noninducer trehalose at 3.1 A resolution. The model consists of residues 61 to 315 comprising the effector binding domain, which forms a dimer as in other members of the LacI family. This domain is composed of two similar subdomains each consisting of a central beta-sheet sandwiched between alpha-helices. The effector binding pocket is at the interface of these subdomains. In spite of different physiological functions, the crystal structures of the two complexes of TreR turned out to be virtually identical to each other with the conformation being similar to those of the effector binding domains of the LacI and PurR in complex with their effector molecules. According to the crystal structure, the noninducer trehalose binds to a similar site as the trehalose portion of trehalose-6-phosphate. The binding affinity for the former is lower than for the latter. The noninducer trehalose thus binds competitively to the repressor. Unlike the phosphorylated inducer molecule, it is incapable of blocking the binding of the repressor headpiece to its operator DNA. The ratio of the concentrations of trehalose-6-phosphate and trehalose thus is used to switch between the two alternative metabolic uses of trehalose as an osmoprotectant and as a carbon source.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-13718526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-1400170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-1429629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-1549599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-1639817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-1744047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-1904524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-2000410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-2160944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-2215649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-235964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-2649682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-2762299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-2820965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-3173493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-3381086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-3657977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-3885043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-4562709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-4571224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-6402713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-661956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-7553867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-7761829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-7792597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-7932692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-7973627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8011357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8083158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8132474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8188660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8391102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8543068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8563639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8638105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8652549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8692896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8794873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8901866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-8994876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-9148912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-9237914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-9278422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9865945-9301333
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lac Repressors,
http://linkedlifedata.com/resource/pubmed/chemical/LacI protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/PurR protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/PurR protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sugar Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/TreR protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Trehalose,
http://linkedlifedata.com/resource/pubmed/chemical/trehalose repressor protein...,
http://linkedlifedata.com/resource/pubmed/chemical/trehalose-6-phosphate
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0961-8368
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2511-21
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9865945-Crystallization,
pubmed-meshheading:9865945-Escherichia coli,
pubmed-meshheading:9865945-Crystallography, X-Ray,
pubmed-meshheading:9865945-Models, Molecular,
pubmed-meshheading:9865945-Protein Conformation,
pubmed-meshheading:9865945-Bacterial Proteins,
pubmed-meshheading:9865945-Binding Sites,
pubmed-meshheading:9865945-Molecular Sequence Data,
pubmed-meshheading:9865945-Escherichia coli Proteins,
pubmed-meshheading:9865945-Repressor Proteins
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