Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-3-18
pubmed:abstractText
Repetitive sequences have been proposed to be recombinogenic elements in eukaryotic chromosomes. We tested whether dinucleotide repeats sequences are preferential sites for recombination because of their high affinity for recombination enzymes. We compared the kinetics of the binding of the scRad51, hsRad51 and ecRecA proteins to oligonucleotides with repeats of dinucleotides GT, CA, CT, GA, GC or AT. Since secondary structures in single-stranded DNA (ssDNA) act as a barrier to complete binding we measured whether these oligonucleotides are able to form stable secondary structures. We show that the preferential binding of recombination proteins is conserved among the three proteins and is influenced mainly by secondary structures in ssDNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
596-600
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9862985-Base Sequence, pubmed-meshheading:9862985-Conserved Sequence, pubmed-meshheading:9862985-DNA, Single-Stranded, pubmed-meshheading:9862985-DNA-Binding Proteins, pubmed-meshheading:9862985-Dinucleotide Repeats, pubmed-meshheading:9862985-Escherichia coli, pubmed-meshheading:9862985-Hot Temperature, pubmed-meshheading:9862985-Humans, pubmed-meshheading:9862985-Kinetics, pubmed-meshheading:9862985-Nucleic Acid Conformation, pubmed-meshheading:9862985-Nucleic Acid Denaturation, pubmed-meshheading:9862985-Protein Binding, pubmed-meshheading:9862985-Rad51 Recombinase, pubmed-meshheading:9862985-Rec A Recombinases, pubmed-meshheading:9862985-Recombination, Genetic, pubmed-meshheading:9862985-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:9862985-Saccharomyces cerevisiae, pubmed-meshheading:9862985-Saccharomyces cerevisiae Proteins
pubmed:year
1999
pubmed:articleTitle
Conserved sequence preference in DNA binding among recombination proteins: an effect of ssDNA secondary structure.
pubmed:affiliation
Institut Curie, Section de Recherche UMR144-CNRS, 26 rue d'Ulm, F-75248-Paris Cedex 05, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't