|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
1999-1-11
|
|
pubmed:abstractText |
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine involved in haematopoiesis and host defence. Production of GM-CSF has been detected in tumour cells including the U87MG astrocytoma cell line. Previous studies have been focused on the regulatory role of the proximal region of the GM-CSF promoter. Our studies on the distal region of the promoter in U87MG cells identify a negative cis element (-1377/-1298) which contains a AP1-like site able to bind c-jun and c-fos transcription factors, according to the results of DNA/protein binding assays. Mutagenesis of the AP1-like site eliminates AP1 binding and the negative effect on promoter activity.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
27
|
|
pubmed:volume |
440
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
119-24
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:9862439-Antibodies,
pubmed-meshheading:9862439-Binding, Competitive,
pubmed-meshheading:9862439-Consensus Sequence,
pubmed-meshheading:9862439-DNA Footprinting,
pubmed-meshheading:9862439-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9862439-Genes, Reporter,
pubmed-meshheading:9862439-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9862439-Humans,
pubmed-meshheading:9862439-Oligonucleotides,
pubmed-meshheading:9862439-Point Mutation,
pubmed-meshheading:9862439-Promoter Regions, Genetic,
pubmed-meshheading:9862439-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:9862439-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:9862439-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9862439-Sequence Deletion,
pubmed-meshheading:9862439-Transcription Factor AP-1,
pubmed-meshheading:9862439-Transfection,
pubmed-meshheading:9862439-Tumor Cells, Cultured
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
An upstream negative regulatory element in human granulocyte-macrophage colony-stimulating factor promoter is recognised by AP1 family members.
|
|
pubmed:affiliation |
Department of Clinical and Experimental Oncology, University of Genoa, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
