Linked Life Data

9858678

Source:http://linkedlifedata.com/resource/pubmed/id/9858678

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-3-4
pubmed:abstractText
Eyelid fusion normally occurs between E15.5 and E16.5 of mouse embryonic development and results from the migration of a population of periderm-derived epithelial cells over the corneal surface. Cell migration is known to depend on extracellular matrix receptors of the integrin family and to be regulated by growth factors. We were therefore interested that a failure of eyelid fusion has been reported in mice that are homozygous null for the transforming growth factor alpha (TGF-alpha) gene and in mice (invalpha5beta1) in which a transgenic alpha5beta1 integrin under the control of the involucrin promoter is misexpressed in differentiating keratinocytes. We examined expression of the alpha2beta1, alpha3beta1, alpha5beta1 and alpha6beta4 integrins during eyelid fusion in wild-type embryos and found selective upregulation of the alpha5beta1 integrin and its ligand, fibronectin, in the migrating eyelid tip cells. In TGF-alpha null embryos, the failure of eyelid fusion was correlated with a failure to upregulate the alpha5beta1 integrin and fibronectin in the tip cells. Using beta-galactosidase as a reporter gene in transgenic mice, we observed specific activity of the involucrin promoter in the eyelid tip cells. In invalpha5beta1 mice the transgenic human integrin was overexpressed not only in the tip cells but throughout the eyelid epidermis. In contrast, the endogenous, murine, alpha5beta1 integrin was only weakly expressed in the tip cells. We speculate that selective and coordinated expression of the alpha5beta1 integrin and fibronectin in eyelid tip cells is required for eyelid fusion and may be under the control of growth factors that include TGF-alpha.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0925-4773
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Elsevier Science Ireland Ltd. All Rights Reserved
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37-45
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9858678-Animals, pubmed-meshheading:9858678-Antigens, Surface, pubmed-meshheading:9858678-Cell Movement, pubmed-meshheading:9858678-Epidermis, pubmed-meshheading:9858678-Eye Abnormalities, pubmed-meshheading:9858678-Eyelids, pubmed-meshheading:9858678-Female, pubmed-meshheading:9858678-Humans, pubmed-meshheading:9858678-Integrin alpha3beta1, pubmed-meshheading:9858678-Integrin alpha6beta4, pubmed-meshheading:9858678-Integrins, pubmed-meshheading:9858678-Male, pubmed-meshheading:9858678-Mice, pubmed-meshheading:9858678-Mice, Inbred C57BL, pubmed-meshheading:9858678-Mice, Knockout, pubmed-meshheading:9858678-Morphogenesis, pubmed-meshheading:9858678-Promoter Regions, Genetic, pubmed-meshheading:9858678-Protein Precursors, pubmed-meshheading:9858678-Receptors, Collagen, pubmed-meshheading:9858678-Receptors, Fibronectin, pubmed-meshheading:9858678-Recombinant Fusion Proteins, pubmed-meshheading:9858678-Transforming Growth Factor alpha
pubmed:year
1998
pubmed:articleTitle
Role of integrins in mouse eyelid development: studies in normal embryos and embryos in which there is a failure of eyelid fusion.
pubmed:affiliation
Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't