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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1999-2-10
|
| pubmed:abstractText |
Quantitative detection of hepatitis B virus (HBV) in serum or plasma is of significance for monitoring of therapy and establishment of the prognosis of the disease, as well as for infectivity assessment and quality control of the diagnosis. Unfortunately, various commercially available test kits for HBV DNA yielded conflicting quantitative results, with differences of up to a factor of 120. The Eurohep Pathobiology Group has established two reference samples of plasma from HBV carriers and determined as accurately as possible the number of HBV DNA molecules in these samples. Plasma donations from two single highly viremic carriers of HBV genotype A (HBV surface antigen subtype adw2) and genotype D (ayw2/3), respectively, were collected, and coded dilutions of these samples were analyzed by members of the Eurohep Pathobiology Group. Quantitative results from the seven laboratories reporting consistent results were initially divergent. Limiting dilution and nested PCR assays suffered from incomplete DNA extraction. Hybridization assays used inaccurately quantitated cloned DNA as a reference. Two hybridization assays could not be calibrated directly with cloned HBV DNA, because virion-derived DNA reacted much less efficiently. After identification and elimination of these problems, limiting-dilution assays from three laboratories and hybridization assays from two producers generated consistent and concordant results: 2.7 x 10(9) HBV DNA molecules/ml (range, 2.1 x 10(9) to 3.4 x 10(9) HBV DNA molecules/ml) in the plasma from the carrier of genotype A and 2.6 x 10(9) HBV DNA molecules/ml (range, 2.1 x 10(9) to 3.0 x 10(9) HBV DNA molecules/ml in the plasma from the carrier of genotype D. The two Eurohep reference plasma samples have already been used for the standardization of test kits and in quality control trials, and the plasma from the carrier of genotype A will probably be the basis of a World Health Organization reference sample.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-1556163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-2656908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-2732516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-3743555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-7061996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-7438207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-7572814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-7699047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-7714058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-7814529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-8202389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-8228921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-8501209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-8633078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-8666521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-8818875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9854066-908854
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0095-1137
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
68-73
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1999
|
| pubmed:articleTitle |
Quantitative detection of hepatitis B virus DNA in two international reference plasma preparations. Eurohep Pathobiology Group.
|
| pubmed:affiliation |
Division of Medical Microbiology and National Reference Laboratory for Viral Hepatitis, University of Gottingen, D 37075 Gottingen, Germany.
|
| pubmed:publicationType |
Journal Article
|