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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
51
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pubmed:dateCreated |
1999-1-26
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pubmed:databankReference |
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pubmed:abstractText |
Recently, the growth hormone secretagogue receptor (GHS-R) cDNA has been isolated from the pituitary and hypothalamus. To evaluate the regulation of human (h) GHS-R gene expression, we cloned the hGHS-R gene containing the 5'-flanking region of 0.6-2.9 kilobase pairs. Analysis of the hGHS-R transcripts with 5'-rapid amplification of cDNA ends suggested that the putative transcription initiation site was approximately -453 base pairs upstream of the translation initiation site (+1). There is no typical TATA, CAAT, or GC box but an initiator-like sequence and putative binding sites for several transcription factors around the putative transcription start site. The 5'-flanking region inserted into a luciferase reporter vector had promoter activity in GH3 cells but had activity indistinguishable from background in HeLa or EP1 cells. The hGHS-R promoter activity in GH3 cells increased by deletion of nucleotides from -1224 to -734, whereas it was decreased by further deletion from -734 to -608. Knowledge of the promoter region of the hGHS-R gene will facilitate elucidation of its transcriptional control.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33885-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9852035-Base Sequence,
pubmed-meshheading:9852035-Cloning, Molecular,
pubmed-meshheading:9852035-GTP-Binding Proteins,
pubmed-meshheading:9852035-Gene Expression Regulation,
pubmed-meshheading:9852035-Genomic Library,
pubmed-meshheading:9852035-HeLa Cells,
pubmed-meshheading:9852035-Humans,
pubmed-meshheading:9852035-Molecular Sequence Data,
pubmed-meshheading:9852035-Pituitary Gland,
pubmed-meshheading:9852035-Promoter Regions, Genetic,
pubmed-meshheading:9852035-Protein Biosynthesis,
pubmed-meshheading:9852035-Random Amplified Polymorphic DNA Technique,
pubmed-meshheading:9852035-Receptors, Cell Surface,
pubmed-meshheading:9852035-Receptors, G-Protein-Coupled,
pubmed-meshheading:9852035-Receptors, Ghrelin,
pubmed-meshheading:9852035-Recombinant Proteins,
pubmed-meshheading:9852035-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9852035-Restriction Mapping,
pubmed-meshheading:9852035-Sequence Deletion,
pubmed-meshheading:9852035-Transcription, Genetic,
pubmed-meshheading:9852035-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
Cloning and characterization of the 5'-flanking region of the human growth hormone secretagogue receptor gene.
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pubmed:affiliation |
Third Division, Department of Medicine, Kobe University School of Medicine, Kobe 650-0017, Japan. hkaji@med.kobe-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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