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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
|
pubmed:dateCreated |
1999-1-7
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pubmed:abstractText |
A rapid method combining flow cytometry and immunomagnetic bead separation (IMFC) was effective for detecting low populations of Escherichia coli O157:H7 inoculated into ground beef, apple juice, and raw milk samples. Modified buffered peptone was most effective for enrichment of E. coli O157:H7 in ground beef. Using IMFC in combination with a 6-h enrichment, as few as four E. coli O157:H7 cells/g of ground beef were detected in as little as a 7-h total analysis time. Our results demonstrate the possibility of using flow cytometry and immunomagnetic bead separation to detect low concentrations of E. coli O157:H7 in ground beef, apple juice and milk.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
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pubmed:issn |
0168-1605
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
20
|
pubmed:volume |
44
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
115-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9849789-Animals,
pubmed-meshheading:9849789-Beverages,
pubmed-meshheading:9849789-Cattle,
pubmed-meshheading:9849789-Escherichia coli,
pubmed-meshheading:9849789-Flow Cytometry,
pubmed-meshheading:9849789-Hydrogen-Ion Concentration,
pubmed-meshheading:9849789-Immunomagnetic Separation,
pubmed-meshheading:9849789-Meat,
pubmed-meshheading:9849789-Milk,
pubmed-meshheading:9849789-Rosales
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pubmed:year |
1998
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pubmed:articleTitle |
Rapid detection of Escherichia coli O157:H7 using immuno-magnetic flow cytometry in ground beef, apple juice, and milk.
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pubmed:affiliation |
Center for Food Safety and Quality Enhancement, Food Science & Technology, University of Georgia, Griffin 30223-1797, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|