Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-1-28
pubmed:abstractText
Microglia are the main human immunodeficiency virus (HIV) reservoir in the central nervous system and most likely play a major role in the development of HIV dementia (HIVD). To characterize human adult microglial chemokine receptors, we analyzed the expression and calcium signaling of CCR5, CCR3, and CXCR4 and their roles in HIV entry. Microglia expressed higher levels of CCR5 than of either CCR3 or CXCR4. Of these three chemokine receptors, only CCR5 and CXCR4 were able to transduce a signal in microglia in response to their respective ligands, MIP-1beta and SDF-1alpha, as recorded by single-cell calcium flux experiments. We also found that CCR5 is the predominant coreceptor used for infection of human adult microglia by the HIV type 1 dementia isolates HIV-1DS-br, HIV-1RC-br, and HIV-1YU-2, since the anti-CCR5 antibody 2D7 was able to dramatically inhibit microglial infection by both wild-type and single-round luciferase pseudotype reporter viruses. Anti-CCR3 (7B11) and anti-CXCR4 (12G5) antibodies had little or no effect on infection. Last, we found that virus pseudotyped with the DS-br and RC-br envelopes can infect cells transfected with CD4 in conjunction with the G-protein-coupled receptors APJ, CCR8, and GPR15, which have been previously implicated in HIV entry.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-10195226, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-1830110, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-2223238, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-3094962, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-3277272, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-3464691, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-7101727, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-7544376, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-7851889, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8294032, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8599205, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8602761, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8629022, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8649511, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-87477, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8892885, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8929542, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8971794, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8972425, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8977299, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8987784, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8995603, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-8996832, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9005985, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9024664, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9106073, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9151905, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9166430, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9179782, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9230441, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9236192, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9281497, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9327737, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9371556, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9405683, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9417093, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9420285, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9520995, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9525605, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9525662, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9537902, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9557714, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9573226, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9573265, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9621075, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9733831, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9744279, http://linkedlifedata.com/resource/pubmed/commentcorrection/9847323-9791028
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-13
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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