Linked Life Data

9846609

Source:http://linkedlifedata.com/resource/pubmed/id/9846609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1999-1-19
pubmed:abstractText
Mice were immunised three to five times with extracts of Anopheles stephensi heads, midguts, ovaries or fat bodies. At each immunisation the effects of feeding An. stephensi on the mice was determined, and changes in mosquito longevity and fecundity examined as the immune response developed. Although variability was common between control cages, significant and consistent reductions in mosquito longevity were observed when midguts were used as immunogens. Other extracts caused transient reductions in mortality. Fecundity was reduced significantly in mosquitoes fed upon mice immunised with each extract in at least one experiment. Mosquitoes fed upon fat-body-immunised mice showed delayed egg-laying as well as overall reduction in fecundity. The results confirm the feasibility of targeting mosquito antigens for novel vaccine development, but the "shotgun" approach used probably fails to successfully hit a suitable target antigen with any consistency. The natural variation in mosquito mortality can be countered by rigorous statistical analysis which can identify subtle effects in a very "noisy" experimental system. The midgut is the obvious target organ for anti-mosquito vaccine development and future work will focus on targeting components of this tissue for further immunisations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0020-7519
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1721-31
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Induced immunity against the mosquito Anopheles stephensi Liston (Diptera: Culicidae): effects on mosquito survival and fecundity.
pubmed:affiliation
Department of Biology, Imperial College of Science, Technology and Medicine, London, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't