Linked Life Data

9845676

Source:http://linkedlifedata.com/resource/pubmed/id/9845676

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-2-8
pubmed:abstractText
During pregnancy, the decidua is comprised of two separate tissues located either mesometrially or antimesometrially in the uterus. Trophoblast invasion takes place only in the mesometrial decidua, where extensive angiogenesis, essential for successful implantation, occurs. Both basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) have been implicated in this phenomenon. The aim of this study was to determine whether the expression of both growth factors is intrinsic to decidua and occurs in the absence of conceptuses, whether their genes are expressed specifically in the mesometrial decidua, the site of angiogenesis, and whether both growth factors are developmentally and hormonally regulated. Decidual tissue was dissected from pseudopregnant rats and levels of both bFGF and VEGF mRNA were examined in mesometrial and antimesometrial decidua by semi-quantitative RT-PCR at different stages of pseudopregnancy. Although induction of decidualization triggered the mRNA expression of bFGF, VEGF mRNA expression remained unchanged. VEGF mRNA level was similar in both antimesometrial and mesometrial decidua, and remained constant throughout pseudopregnancy. In sharp contrast, bFGF mRNA was highly expressed in the mesometrial decidua at a time when extensive angiogenesis takes place in this tissue. Very little signal was observed in the antimesometrial decidua. To examine the regulation of these growth factors, we used a temperature-sensitive decidual cell line developed by transforming antimesometrial decidual cells with SV-40 tsA 209 mutant virus. These cells express both bFGF and VEGF mRNA. Because progesterone is necessary for decidualization and decidua secretes prolactin (PRL)-related hormones, we examined the role of these hormones on VEGF and bFGF mRNA expressions. Neither progesterone nor PRL had any effect on VEGF mRNA levels. However, bFGF mRNA expression was greatly stimulated by PRL. In conclusion, results of this investigation have revealed that bFGF, but not VEGF, mRNA becomes highly expressed in the mesometrial decidua, where angiogenesis occurs, and where trophoblasts, by invading decidual cells, may promote the release of bFGF. In addition, these results indicate that the locally secreted PRL-like hormone up-regulates the mRNA expression of bFGF.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0952-5041
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
355-62
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9845676-Animals, pubmed-meshheading:9845676-Base Sequence, pubmed-meshheading:9845676-Cell Line, pubmed-meshheading:9845676-DNA Primers, pubmed-meshheading:9845676-Decidua, pubmed-meshheading:9845676-Endothelial Growth Factors, pubmed-meshheading:9845676-Female, pubmed-meshheading:9845676-Fibroblast Growth Factor 2, pubmed-meshheading:9845676-Gene Expression Regulation, Developmental, pubmed-meshheading:9845676-Lymphokines, pubmed-meshheading:9845676-Neovascularization, Physiologic, pubmed-meshheading:9845676-Pregnancy, pubmed-meshheading:9845676-Progesterone, pubmed-meshheading:9845676-Prolactin, pubmed-meshheading:9845676-Pseudopregnancy, pubmed-meshheading:9845676-RNA, Messenger, pubmed-meshheading:9845676-Rats, pubmed-meshheading:9845676-Rats, Sprague-Dawley, pubmed-meshheading:9845676-Vascular Endothelial Growth Factor A, pubmed-meshheading:9845676-Vascular Endothelial Growth Factors
pubmed:year
1998
pubmed:articleTitle
Developmental expression and regulation of basic fibroblast growth factor and vascular endothelial growth factor in rat decidua and in a decidual cell line.
pubmed:affiliation
Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.