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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1998-12-28
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pubmed:databankReference |
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pubmed:abstractText |
The mechanism by which the phytochrome (phy) photoreceptor family transduces informational light signals to photoresponsive genes is unknown. Using a yeast two-hybrid screen, we have identified a phytochrome-interacting factor, PIF3, a basic helix-loop-helix protein containing a PAS domain. PIF3 binds to wild-type C-terminal domains of both phyA and phyB, but less strongly to signaling-defective, missense mutant-containing domains. Expression of sense or antisense PIF3 sequences in transgenic Arabidopsis perturbs photoresponsiveness in a manner indicating that PIF3 functions in both phyA and phyB signaling pathways in vivo. PIF3 localized to the nucleus in transient transfection experiments, indicating a potential role in controlling gene expression. Together, the data suggest that phytochrome signaling to photoregulated genes includes a direct pathway involving physical interaction between the photoreceptor and a transcriptional regulator.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/PHYA protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/PHYB protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Phytochrome,
http://linkedlifedata.com/resource/pubmed/chemical/Phytochrome A,
http://linkedlifedata.com/resource/pubmed/chemical/Phytochrome B,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
657-67
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9845368-Arabidopsis,
pubmed-meshheading:9845368-Arabidopsis Proteins,
pubmed-meshheading:9845368-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:9845368-Cell Nucleus,
pubmed-meshheading:9845368-DNA, Plant,
pubmed-meshheading:9845368-Helix-Loop-Helix Motifs,
pubmed-meshheading:9845368-Molecular Sequence Data,
pubmed-meshheading:9845368-Oligonucleotides, Antisense,
pubmed-meshheading:9845368-Photoreceptor Cells,
pubmed-meshheading:9845368-Phytochrome,
pubmed-meshheading:9845368-Phytochrome A,
pubmed-meshheading:9845368-Phytochrome B,
pubmed-meshheading:9845368-Plants, Genetically Modified,
pubmed-meshheading:9845368-Sequence Homology, Amino Acid,
pubmed-meshheading:9845368-Signal Transduction,
pubmed-meshheading:9845368-Transcription Factors,
pubmed-meshheading:9845368-Transfection,
pubmed-meshheading:9845368-Yeasts
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pubmed:year |
1998
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pubmed:articleTitle |
PIF3, a phytochrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein.
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pubmed:affiliation |
Department of Plant and Microbial Biology, University of California, Berkeley 94720, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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