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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1999-2-4
|
| pubmed:abstractText |
Digestion of bovine alpha s1-casein with bovine trypsin produced peptide(s) with an inhibitory effect on concanavalin A-induced proliferation of mouse spleen cells. One of these peptides was isolated from the alpha s1-casein digest following ultrafiltration, hydroxyapatite chromatography and reversed-phase HPLC, and amino acid composition and sequence analyses showed it to be sequence 59-79 from the phosphoserine-rich region of alpha s1-casein. The isolated peptide significantly inhibited the concanavalin A-induced proliferation of mouse spleen cells and rabbit Peyer's patch cells, whereas it enhanced the lipopolysaccharide- and phytohaemagglutinin-induced proliferation of both cells. The peptide displayed mitogenic activity in the cell cultures without the commercial mitogen, and significantly enhanced immunoglobulin production. Moreover, residues 1-25 from the phosphoserine-rich region of bovine beta-casein had a similar effect on the proliferation of mouse spleen cells and rabbit Peyer's patch cells stimulated or not stimulated by the commercial mitogen. These results indicate that caseinophosphopeptides may act as a humoral immunostimulator in cell cultures.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Caseins,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-0299
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
569-78
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9839212-Amino Acid Sequence,
pubmed-meshheading:9839212-Amino Acids,
pubmed-meshheading:9839212-Animals,
pubmed-meshheading:9839212-Caseins,
pubmed-meshheading:9839212-Cattle,
pubmed-meshheading:9839212-Cell Division,
pubmed-meshheading:9839212-Cells, Cultured,
pubmed-meshheading:9839212-Concanavalin A,
pubmed-meshheading:9839212-Female,
pubmed-meshheading:9839212-Immunoglobulins,
pubmed-meshheading:9839212-Lymphocytes,
pubmed-meshheading:9839212-Male,
pubmed-meshheading:9839212-Mice,
pubmed-meshheading:9839212-Mice, Inbred C3H,
pubmed-meshheading:9839212-Molecular Sequence Data,
pubmed-meshheading:9839212-Peptide Fragments,
pubmed-meshheading:9839212-Phosphopeptides,
pubmed-meshheading:9839212-Rabbits,
pubmed-meshheading:9839212-Spleen,
pubmed-meshheading:9839212-Trypsin
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Identification of a phosphopeptide in bovine alpha s1-casein digest as a factor influencing proliferation and immunoglobulin production in lymphocyte cultures.
|
| pubmed:affiliation |
Laboratory of Food Bioscience, Faculty of Agriculture, Shinshu University, Nagano-ken, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|