Linked Life Data

9836596

Source:http://linkedlifedata.com/resource/pubmed/id/9836596

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
48
pubmed:dateCreated
1999-1-7
pubmed:abstractText
Calcium is required for the binding and endocytosis of protein ligands by the low-density lipoprotein receptor-related protein (LRP) and other members of the low-density lipoprotein (LDL) receptor family. Calcium binding sites are thought to be present in the complement-like repeats that occur in clusters in all members of this receptor family. We have expressed two such complement-like repeats, CR3 and CR8, from an alpha2-macroglobulin-proteinase ligand binding region of LRP, as well as repeat 1 from the LDL receptor and examined the metal binding properties and resulting structural changes of these three repeats using changes in tryptophan and terbium fluorescence and perturbation of [1H-15N]-HSQC NMR spectra of the 15N-labeled domains from LRP. We found that all three domains contain a tight calcium binding site at physiological pH and that calcium binding results in a major structural rigidification. Changes in tryptophan fluorescence and tryptophan-sensitized terbium fluorescence indicate that the calcium binding sites are located in homologous regions in all of the repeats. Differences in the details of the perturbations, as well as in the pH dependence of calcium binding, show, however, that each metal site is distinct.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17016-23
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9836596-Amino Acid Sequence, pubmed-meshheading:9836596-Calcium, pubmed-meshheading:9836596-Calcium-Binding Proteins, pubmed-meshheading:9836596-Complement System Proteins, pubmed-meshheading:9836596-Fluorescence, pubmed-meshheading:9836596-Gadolinium, pubmed-meshheading:9836596-Humans, pubmed-meshheading:9836596-Hydrogen-Ion Concentration, pubmed-meshheading:9836596-Low Density Lipoprotein Receptor-Related Protein-1, pubmed-meshheading:9836596-Molecular Sequence Data, pubmed-meshheading:9836596-Nuclear Magnetic Resonance, Biomolecular, pubmed-meshheading:9836596-Peptide Fragments, pubmed-meshheading:9836596-Receptors, Immunologic, pubmed-meshheading:9836596-Receptors, LDL, pubmed-meshheading:9836596-Recombinant Proteins, pubmed-meshheading:9836596-Repetitive Sequences, Amino Acid, pubmed-meshheading:9836596-Terbium, pubmed-meshheading:9836596-Tryptophan
pubmed:year
1998
pubmed:articleTitle
Characterization of the calcium site in two complement-like domains from the low-density lipoprotein receptor-related protein (LRP) and comparison with a repeat from the low-density lipoprotein receptor.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612-4316, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't