Linked Life Data

9831929

Source:http://linkedlifedata.com/resource/pubmed/id/9831929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-3-25
pubmed:abstractText
1. In the present work, we study the effect of NO on the proliferation and differentiation of brown fat cells in primary cultures. 2. Brown fat precursor cells isolated from rat brown adipose tissue were cultured for 8 days until confluence and treated daily with the NO donating agents, S-nitroso-acetyl penicillamine (SNAP) or S-nitroso-L-glutathione (GSNO). Both agents (300 microM) decreased cell proliferation approximately 8 fold on day 8. The inhibitory effect of NO was unlikely to be due to cytotoxicity since (i) cells never completely lost their proliferation capacity even after 8 days of exposure to repeated additions of SNAP or GSNO, and (ii) the inhibitory effect was reversible after removal of the media containing NO donors. 3. Daily treatment with nitric oxide synthase inhibitors, such as NG-nitro-L-arginine methyl ester (L-NAME, 300 microM), led to the stimulation of cell proliferation by 44+/-5%, n=3, suggesting that NO, endogenously produced in brown adipocytes, may be involved in modulating cell growth. 4. Daily treatment with both SNAP or GSNO induced significant mitochondriogenesis, measured as the mitochondrial conversion of 3-[4,5-dimethylthiazol-2-yl-]-2,5-diphenyl tetrazolium bromide (MTT) to formazan, whilst daily treatment with L-NAME was without effect. 5. The inhibition of cell proliferation by NO donors was accompanied by the expression of two genes coding for peroxisome proliferator activated receptor-gamma and uncoupling protein-1, which are upregulated during differentiation. 6. Increasing cyclic GMP in cells by 8-bromo-cyclic GMP (100-1000 microM) did not reproduce the observed NO effects on either cell number or gene expression. On the other hand, chronic treatment with the inhibitor of the NO-stimulated guanylyl cyclase, 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ), reduced the expression of peroxisome proliferator activated receptor-gamma and uncoupling protein-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
888-94
pubmed:dateRevised
2008-11-20
pubmed:meshHeading
pubmed-meshheading:9831929-Adipose Tissue, Brown, pubmed-meshheading:9831929-Animals, pubmed-meshheading:9831929-Base Sequence, pubmed-meshheading:9831929-Cell Differentiation, pubmed-meshheading:9831929-Cell Division, pubmed-meshheading:9831929-Cells, Cultured, pubmed-meshheading:9831929-Cyclic GMP, pubmed-meshheading:9831929-Glutathione, pubmed-meshheading:9831929-Male, pubmed-meshheading:9831929-Molecular Sequence Data, pubmed-meshheading:9831929-NG-Nitroarginine Methyl Ester, pubmed-meshheading:9831929-Nitric Oxide, pubmed-meshheading:9831929-Nitric Oxide Synthase, pubmed-meshheading:9831929-Nitroso Compounds, pubmed-meshheading:9831929-Penicillamine, pubmed-meshheading:9831929-Radioimmunoprecipitation Assay, pubmed-meshheading:9831929-Rats, pubmed-meshheading:9831929-Rats, Sprague-Dawley, pubmed-meshheading:9831929-S-Nitrosoglutathione, pubmed-meshheading:9831929-Time Factors
pubmed:year
1998
pubmed:articleTitle
Effects of nitric oxide on proliferation and differentiation of rat brown adipocytes in primary cultures.
pubmed:affiliation
Center for Study and Research on Obesity, Department of Pharmacology, Chemotherapy and Medical Toxicology, School of Medicine, LITA Vialba, L. Sacco Hospital, University of Milan, Milano, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't