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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-12-7
|
| pubmed:abstractText |
The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0026-8925
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
260
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
92-101
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:9829832-Chromosomes, Artificial, Yeast,
pubmed-meshheading:9829832-Cloning, Molecular,
pubmed-meshheading:9829832-Contig Mapping,
pubmed-meshheading:9829832-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:9829832-Fungi,
pubmed-meshheading:9829832-Gene Library,
pubmed-meshheading:9829832-Genes, Plant,
pubmed-meshheading:9829832-Hordeum,
pubmed-meshheading:9829832-Plant Diseases,
pubmed-meshheading:9829832-Polymerase Chain Reaction,
pubmed-meshheading:9829832-Recombination, Genetic,
pubmed-meshheading:9829832-Selection, Genetic
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Chromosome landing at the barley Rar1 locus.
|
| pubmed:affiliation |
The Sainsbury Laboratory, John Innes Centre Norwich Research Park, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|