|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1998-12-17
|
|
pubmed:abstractText |
Organophosphorus (OP) insecticides and nerve agents that contain P-S bond are relatively more resistant to enzymatic hydrolysis. Purified phenol oxidase (laccase) from the white rot fungus Pleurotus ostreatus (Po) together with the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) displayed complete and rapid oxidative degradation of the nerve agents VX and Russian VX (RVX) and the insecticide analog diisopropyl-Amiton with specific activity: k(sp) = 2200, 667 and 1833 nmol min(-1) mg(-1), respectively (pH 7.4, 37 degrees C). A molar ratio of 1:20 for OP/ABTS and 0.05 M phosphate at pH 7.4 provided the highest degradation rate of VX and RVX. The thermostable laccase purified from the fungus Chaetomium thermophilium (Ct) in the presence of ABTS caused a 52-fold slower degradation of VX with k(sp) = 42 nmol min(-1) mg(-1). The enzymatic biodegradation products were identified by 31P-NMR and GC/MS analysis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
6
|
|
pubmed:volume |
438
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
195-200
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:9827544-Benzothiazoles,
pubmed-meshheading:9827544-Biodegradation, Environmental,
pubmed-meshheading:9827544-Insecticides,
pubmed-meshheading:9827544-Kinetics,
pubmed-meshheading:9827544-Laccase,
pubmed-meshheading:9827544-Organophosphorus Compounds,
pubmed-meshheading:9827544-Organothiophosphorus Compounds,
pubmed-meshheading:9827544-Oxidation-Reduction,
pubmed-meshheading:9827544-Oxidoreductases,
pubmed-meshheading:9827544-Pleurotus,
pubmed-meshheading:9827544-Substrate Specificity,
pubmed-meshheading:9827544-Sulfonic Acids
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
Oxidative biodegradation of phosphorothiolates by fungal laccase.
|
|
pubmed:affiliation |
Department of Pharmacology, Israel Institute for Biological Research, Ness Ziona. amitai@iibr.gov.il
|
|
pubmed:publicationType |
Journal Article
|
