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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-12-30
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pubmed:abstractText |
The mitochondrial uncoupling protein UCP-1 uncouples respiration from ATP synthesis in brown adipose tissue (BAT) and thus energy is dissipated as heat. Recently two further isoforms have been identified which may play a similar role in other tissues. We have determined the effects of the rodent-selective beta3-adrenoceptor (beta3-AR) agonist BRL 35135, on beta3-AR and UCP mRNA levels in tissues from lean and obese (fa/fa) Zucker rats. beta3-AR mRNA levels were reduced in fa/fa white (WAT) and brown (BAT) adipose tissue relative to levels in lean littermates. BRL 35135 treatment increased expression levels of beta3-AR mRNA in both genotypes. UCP-2 and UCP-3 mRNA levels in BAT, WAT and skeletal muscle were reduced by 2-3 fold in the fa/fa rats relative to the lean rats. We confirm that BRL 35135 increases BAT UCP-1 mRNA in lean rats, and find that BAT UCP-3 mRNA was reduced 3.2 fold, with no changes in UCP-2 expression. In WAT BRL 35135 increased UCP-2 and UCP-3 expression 2-3 fold in both lean and fa/fa rats. In lean rats, skeletal muscle UCP-3 mRNA was increased 2.3 fold by BRL 35135 whereas UCP-2 was reduced by 2.2 fold. BRL 35135 had no effects on UCP-2 and UCP-3 expression in skeletal muscle of the fa/fa rats. Our results demonstrate that mechanisms regulating UCP isoform synthesis in fa/fa rats are impaired and that WAT could be involved in the thermogenic response of BRL 35135.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/BRL 35135,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phenethylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/mitochondrial uncoupling protein,
http://linkedlifedata.com/resource/pubmed/chemical/mitochondrial uncoupling protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/mitochondrial uncoupling protein 3
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1998 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
252
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
450-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9826550-Adipose Tissue,
pubmed-meshheading:9826550-Adipose Tissue, Brown,
pubmed-meshheading:9826550-Adrenergic beta-Agonists,
pubmed-meshheading:9826550-Animals,
pubmed-meshheading:9826550-Base Sequence,
pubmed-meshheading:9826550-Body Temperature Regulation,
pubmed-meshheading:9826550-Carrier Proteins,
pubmed-meshheading:9826550-DNA Primers,
pubmed-meshheading:9826550-Gene Expression,
pubmed-meshheading:9826550-Ion Channels,
pubmed-meshheading:9826550-Membrane Proteins,
pubmed-meshheading:9826550-Membrane Transport Proteins,
pubmed-meshheading:9826550-Mitochondrial Proteins,
pubmed-meshheading:9826550-Muscle, Skeletal,
pubmed-meshheading:9826550-Obesity,
pubmed-meshheading:9826550-Phenethylamines,
pubmed-meshheading:9826550-Polymerase Chain Reaction,
pubmed-meshheading:9826550-Proteins,
pubmed-meshheading:9826550-RNA, Messenger,
pubmed-meshheading:9826550-Rats,
pubmed-meshheading:9826550-Rats, Zucker
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pubmed:year |
1998
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pubmed:articleTitle |
The effects of the beta3-adrenoceptor agonist BRL 35135 on UCP isoform mRNA expression.
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pubmed:affiliation |
Clore Laboratory, University of Buckingham, Hunter Street, Buckingham, MK18 1EG, United Kingdom. valur.emilsson@buck.ac.uk
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pubmed:publicationType |
Journal Article
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