Linked Life Data

9825929

Source:http://linkedlifedata.com/resource/pubmed/id/9825929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-1-15
pubmed:abstractText
The immunocytochemical localization of peroxidase in the acinar cells of the Mongolian gerbil parotid gland was studied using aldehyde-fixed tissue prepared by rapid freezing and low-temperature embedding in Lowicryl K4M resin. This freeze-embedding method was developed to provide improved preservation of cell ultrastructure and optimum accessibility of antigens, combined with relative technical ease. Immunogold labeling with antibody to peroxidase was found mainly in the dense cores of mature and immature secretory granules. In the trans Golgi area, the rough endoplasmic reticulum (rER), many clusters of smooth vesicles, and short tubular structures showed a variable labeling intensity. The results demonstrate the utility of this newly-developed preparative method and confirm the presence of peroxidase in elements of the Golgi apparatus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0924-3860
pubmed:author
pubmed:issnType
Print
pubmed:volume
36 Suppl
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
235-9
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Immunogold labeling of peroxidase in salivary gland acinar cells using an improved tissue preparation procedure: rapid freezing and Lowicryl embedding.
pubmed:affiliation
Dept. Oral Histology, Nagasaki University School of Dentistry, Japan.
pubmed:publicationType
Journal Article