Linked Life Data

9824296

Source:http://linkedlifedata.com/resource/pubmed/id/9824296

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-12-10
pubmed:abstractText
Type 2C serine/threonine protein phosphatases (PP2C) so far require unphysiologically large amounts of Mg2+ ions for activity. Activators and inhibitors are not available, targeting subunits unknown. Studying the regulation of PP2C isozymes in bovine retinae, we found that the activity of PP2C increased specifically by the addition of mono- and polyunsaturated fatty acids. Activation was most pronounced at low Mg2+ levels (10-fold stimulation of PP2Calpha by 0.5 mM arachidonic acid at 0.7 mM Mg2+). Sensitivity of PP2Cbeta was 30-50% less, revealing for the first time enzymatic differences among the PP2C isozymes. Combining unsaturated fatty acids with physiological Mg2+ concentrations resulted in PP2C activity that by far exceeded the dephosphorylation rates obtained otherwise. This suggests that PP2C activity has been severely underestimated in the past. In the presence of fatty acids, Ca2+ ions became inhibitory in the micromolar range. We conclude that unsaturated fatty acids may play a role in the regulation of PP2C activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
437
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
229-32
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Protein phosphatase type 2C active at physiological Mg2+: stimulation by unsaturated fatty acids.
pubmed:affiliation
Universität, Institut für Pharmazeutische Chemie, Abteilung Biochemie, Marburg, Germany. klumpp@mailer.uni-marburg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't