Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-12-10
pubmed:databankReference
pubmed:abstractText
A chickpea cDNA encoding a cell wall copper amine oxidase (CuAO) was cloned and characterised. The 2010 bp open reading frame encodes a protein of 76.5 kDa which shares significant primary structure homology with other known CuAOs. Southern blot analysis indicates that in chickpea CuAO is encoded by a single gene or a small gene family. This cDNA was essential for studying the role of CuAO during seedling development and wound healing in chickpea seedlings. CuAO transcript level and activity were modulated during seedling development in parallel with cell maturation. Moreover, mechanical wounding induced a rapid increase of CuAO mRNA accumulation and enzyme activity which remained high during the wound-healing process. Aminoguanidine, a specific CuAO inhibitor, decreased the deposition of lignin-suberin barrier along the lesion. CuAO may be a limiting factor in H2O2 production in the cell wall of chickpea seedlings and its expression seems to integrate with the remodelling of plant cell wall occurring during ontogenesis and wound healing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
437
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-82
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9824285-Amine Oxidase (Copper-Containing), pubmed-meshheading:9824285-Amino Acid Sequence, pubmed-meshheading:9824285-Base Sequence, pubmed-meshheading:9824285-Cell Wall, pubmed-meshheading:9824285-Cloning, Molecular, pubmed-meshheading:9824285-DNA, Complementary, pubmed-meshheading:9824285-Enzyme Activation, pubmed-meshheading:9824285-Fabaceae, pubmed-meshheading:9824285-Gene Expression Regulation, Developmental, pubmed-meshheading:9824285-Gene Expression Regulation, Plant, pubmed-meshheading:9824285-Guanidines, pubmed-meshheading:9824285-Lignin, pubmed-meshheading:9824285-Lipids, pubmed-meshheading:9824285-Membrane Lipids, pubmed-meshheading:9824285-Molecular Sequence Data, pubmed-meshheading:9824285-Plants, pubmed-meshheading:9824285-Plants, Medicinal, pubmed-meshheading:9824285-RNA, Messenger
pubmed:year
1998
pubmed:articleTitle
Developmentally and wound-regulated expression of the gene encoding a cell wall copper amine oxidase in chickpea seedlings.
pubmed:affiliation
Dipartimento di Biologia, Università Roma Tre, Rome, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't