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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
1999-1-7
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pubmed:abstractText |
Biphalin is a bivalent opioid analogue containing two tyrosine residues. We have examined the effect of biphalin's anti-retroviral potency in vitro using a murine model. Biphalin, in non-cytotoxic concentrations, suppressed in a dose-dependent fashion the replication of Friend leukemia virus (FLV) in Mus dunni cells as determined using a focus forming assay. FLV replication was substantially reduced by biphalin at 10(-4) M concentration. When biphalin was combined with 3'-azido-3'-deoxythymidine (AZT) the two acted synergistically in inhibiting FLV replication compared to either used alone. Using a reverse transcriptase (RT) assay, FLV RT levels also were noted to be reduced in the presence of biphalin. These observations indicate that biphalin possesses anti-retroviral activity in vitro, suggesting that this opioid peptide should be examined further in vivo to determine if it is a candidate for combined therapy with AZT and possibly other drugs for retrovirus infections including the human immunodeficiency virus (HIV).
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Zidovudine,
http://linkedlifedata.com/resource/pubmed/chemical/biphalin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0192-0561
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
457-66
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9818790-Antiviral Agents,
pubmed-meshheading:9818790-Cell Line,
pubmed-meshheading:9818790-Dose-Response Relationship, Drug,
pubmed-meshheading:9818790-Enkephalins,
pubmed-meshheading:9818790-Friend murine leukemia virus,
pubmed-meshheading:9818790-Humans,
pubmed-meshheading:9818790-RNA-Directed DNA Polymerase,
pubmed-meshheading:9818790-Virus Replication,
pubmed-meshheading:9818790-Zidovudine
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pubmed:year |
1998
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pubmed:articleTitle |
Inhibitory effect of biphalin and AZT on murine Friend leukemia virus infection in vitro.
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pubmed:affiliation |
Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33612, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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