Linked Life Data

9817880

Source:http://linkedlifedata.com/resource/pubmed/id/9817880

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-12-17
pubmed:abstractText
Two commercial assays that detect Mycobacterium tuberculosis complex (MTB) in clinical specimens by rRNA target amplification (AMTDII) and ligase chain reaction (LCx) were evaluated. The tests were applied to 457 respiratory (n = 273) and extrapulmonary (n = 184) specimens collected from 357 patients. The results were compared with those of acid-fast staining and culture. The combination of culture and clinical diagnosis was considered to be the "gold standard." Seventy specimens were from patients with pulmonary tuberculosis and 28 specimens were from patients with extrapulmonary tuberculosis. After resolution of discrepant results, the overall sensitivities, specificities, and positive and negative predictive values for respiratory specimens were 92.8, 99.4, 98.5, and 97%, respectively, for AMTDII and 75.7, 98.8, 96.4, and 90.5%, respectively, for LCx. With extrapulmonary specimens, the overall sensitivities, specificities, and positive and negative predictive values were 78.6, 99.3, 95.6, and 96.2%, respectively, for AMTDII and 53.6, 99.3, 93.7, and 92.1%, respectively, for LCx. The level of agreement between AMTDII and LCx assay results was 78.2%. We conclude that although both nucleic acid amplification methods are rapid and specific for the detection of MTB in clinical specimens, AMTDII is significantly more sensitive than LCx with both respiratory (P = 0.005) and extrapulmonary (P = 0.048) specimens.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-10215457, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-8815093, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-8922809, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-8929468, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9098609, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9163449, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9230369, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9276432, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9399528, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9508296, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9542930, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817880-9574698
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3601-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Comparative evaluation of the new gen-probe Mycobacterium tuberculosis amplified direct test and the semiautomated abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens.
pubmed:affiliation
Department of Clinical Microbiology, General Hospital Umberto I degrees-Torrette, Ancona, Italy. piersim@tin.it
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't