Linked Life Data

9817873

Source:http://linkedlifedata.com/resource/pubmed/id/9817873

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-12-17
pubmed:abstractText
A recent article (C. G. Thornton et al., J. Clin. Microbiol. 36:1996-2003, 1998) reported a new specimen-processing method for improved recovery of mycobacteria. This method used C18-carboxypropylbetaine (CB-18) and increased both smear and culture sensitivity. The companion article (C. G. Thornton et al., J. Clin. Microbiol. 36:2004-2013, 1998) described initial improvements to this method. Additional significant parameters of the CB-18 processing method are identified herein. First, eliminating the incubation step was shown to further improve culture sensitivity. Subsequently, recovery of several mycobacterial isolates by the CB-18 method was compared to a contemporary processing method that combines NALC and NaOH (NALC-NaOH) and a Tween 80-based method. Recovery of the tuberculous isolates following NALC-NaOH processing averaged 20% and ranged from 1.6 to 45%, whereas recovery of the nontuberculous isolates averaged 11% and ranged from 0.1 to 55%. Recovery of the tuberculous and nontuberculous isolates by the Tween 80-based method ranged from 22 to 92% and 27 to 93%, respectively, with averages of 58 and 65%, respectively. Recovery of the tuberculous and nontuberculous mycobacteria following CB-18 processing averaged 86 and 73%, respectively, with ranges from 61 to over 100% and from 43 to over 100%, respectively. Other parameters of the CB-18 method were also examined, including recovery versus CB-18 concentration and the relationship between CB-18 concentration and the tuberculocidal effect. The tuberculocidal effect was time dependent but independent of concentration, whereas recovery was directly proportional to concentration. Increasing the CB-18 concentration to 4 mM provided quantitative recovery on solid medium; however, higher concentrations of CB-18 were not compatible with liquid culture. Examination of the relationship between increasing CB-18 and lecithin concentrations suggested that lecithin could not overcome the deleterious effects of CB-18 in liquid culture at these higher concentrations.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-13999289, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-14923636, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-14933334, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-3082926, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-4208508, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-4338078, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-4562799, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-459890, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-4961042, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-5321489, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-7000812, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-7539954, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-7665679, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-9154896, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-9650951, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-9650952, http://linkedlifedata.com/resource/pubmed/commentcorrection/9817873-9687483
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3558-66
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
In vitro comparison of NALC-NaOH, tween 80, and C18-carboxypropylbetaine for processing of specimens for recovery of mycobacteria.
pubmed:affiliation
Integrated Research Technology, LLC, Baltimore, Maryland 21227, USA. 104217.456@compuserve.com
pubmed:publicationType
Journal Article, Comparative Study